Detecting the exposure to Cd and PCBs by means of a non-invasive transcriptomic approach in laboratory and wild contaminated European eels (Anguilla anguilla)

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• 作者：Lucie Baillon ; Fabien Pierron ; Jennifer Oses…
• 关键词：cDNA microarray ; Multi ; pollutant ; European eel ; Transcriptomics ; Non ; invasive sampling
• 刊名：Environmental Science and Pollution Research
• 出版年：2016
• 出版时间：March 2016
• 年：2016
• 卷：23
• 期：6
• 页码：5431-5441
• 全文大小：586 KB
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• 作者单位：Lucie Baillon (1) (2)
  Fabien Pierron (1) (2)
  Jennifer Oses (1) (2)
  Pauline Pannetier (3)
  Eric Normandeau (4)
  Patrice Couture (3)
  Pierre Labadie (1) (2)
  Hélène Budzinski (1) (2)
  Patrick Lambert (5)
  Louis Bernatchez (4)
  Magalie Baudrimont (1) (2)
  
  1. Univ. Bordeaux, UMR EPOC CNRS 5805, F-33400, Talence, France
  2. CNRS, EPOC, UMR 5805, F-33400, Talence, France
  3. Institut National de la Recherche Scientifique, Centre Eau Terre Environnement, 490 de la Couronne, Québec, QC, G1K 9A9, Canada
  4. Département de biologie, Institut de Biologie Intégrative et des Systèmes (IBIS), Université Laval, Québec, QC, G1V 0A6, Canada
  5. Irtsea, UR EABX, 50 avenue de Verdun-Gazinet, 33612, Cestas, France
  
• 刊物类别：Earth and Environmental Science
• 刊物主题：Environment
  Environment
  Atmospheric Protection, Air Quality Control and Air Pollution
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Industrial Pollution Prevention
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1614-7499

文摘

Detecting and separating specific effects of contaminants in a multi-stress field context remain a major challenge in ecotoxicology. In this context, the aim of this study was to assess the usefulness of a non-invasive transcriptomic method, by means of a complementary DNA (cDNA) microarray comprising 1000 candidate genes, on caudal fin clips. Fin gene transcription patterns of European eels (Anguilla anguilla) exposed in the laboratory to cadmium (Cd) or a polychloro-biphenyl (PCBs) mixture but also of wild eels from three sampling sites with differing contamination levels were compared to test whether fin clips may be used to detect and discriminate the exposure to these contaminants. Also, transcriptomic profiles from the liver and caudal fin of eels experimentally exposed to Cd were compared to assess the detection sensitivity of the fin transcriptomic response. A similar number of genes were differentially transcribed in the fin and liver in response to Cd exposure, highlighting the detection sensitivity of fin clips. Moreover, distinct fin transcription profiles were observed in response to Cd or PCB exposure. Finally, the transcription profiles of eels from the most contaminated site clustered with those from laboratory-exposed fish. This study thus highlights the applicability and usefulness of performing gene transcription assays on non-invasive tissue sampling in order to detect the in situ exposure to Cd and PCBs in fish.