Characterization of the lipopolysaccharide of <em class="a-plus-plus">Escherichia coliem> 126

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• 作者：L. D. Varbanets ; E. A. Zdorovenko ; O. S. Brovarskaya ; S. V. Kalinichenko
• 关键词：Escherichia coli ; lipopolysaccharide ; biological activity ; О ; specific polysaccharide ; structure
• 刊名：Microbiology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：86
• 期：1
• 页码：56-63
• 全文大小：en>
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Medical Microbiology;
• 出版者：Pleiades Publishing
• ISSN：1608-3237
• 卷排序：86

文摘

The lipopolysaccharide (LPS) of Escherichia coli 126 was isolated and studied. The lipid A fatty acid composition of the investigated LPS was similar to that of other members of the family Enterobacteriaceae. The E. coli 126 LPS was more toxic than the LPSs of previously studied E. coli strains and of other members of the Enterobacteriaceae (Budvicia aquatica and Pragia fontium), and was less pyrogenic than pyrogenal. SDS-PAG electrophoresis showed a bimodal distribution typical of S-form LPSs. The LPS of E. coli 126 decreased the adhesive index indicating a possible competition between LPS molecules of E. coli 126 and adhesins of E. coli F-50 on rabbit erythrocytes. The LPS of E. coli 126 in a homologous system showed antigenic activity in the reactions of double immunodiffusion in agar by Ouchterlony. No serological cross-reaction of the LPS of other E. coli strains, as well as of that of the B. aquatica type strain, with the antiserum to E. coli 126 was observed. The structural components of the lipopolysaccharide obtained by mild acid hydrolysis were lipid A, the core oligosaccharide, and the O-specific polysaccharide. Based on the data of monosaccharide analysis and 1H and 13C NMR spectroscopy it was found that the O-specific polysaccharide had the structure characteristic of the representatives of E. coli serogroup O15.