Conditional anterograde tracing reveals distinct targeting of individual serotonin cell groups (B5–B9) to the forebrain and brainstem
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- 作者：Aude Muzerelle ; Sophie Scotto-Lomassese…
- 关键词：Genetic mouse models ; Anatomical tract ; tracing ; GFP ; SERT ; Serotonin ; Tegmental nucleus ; Pearson correlation ; Olfactory bulb ; Habenula ; Prefrontal cortex ; Median raphe ; Dorsal raphe ; Hippocampus ; Amygdala ; Axon tracing ; AAV
- 刊名：Brain Structure and Function
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：221
- 期：1
- 页码：535-561
- 全文大小：12,166 KB
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Sophie Scotto-Lomassese (1) (2) (3)
Jean François Bernard (2) (4)
Mariano Soiza-Reilly (1) (2) (3)
Patricia Gaspar (1) (2) (3)
1. Inserm UMR-S 839, 17 rue du Fer à Moulin, 75005, Paris, France
2. Université Pierre et Marie Curie, Paris, France
3. Institut du Fer à Moulin, Paris, France
4. Inserm UMR- 894, Centre Psychiatrie St Anne, Paris, France - 刊物主题：Neurosciences; Cell Biology; Neurology;
- 出版者：Springer Berlin Heidelberg
- ISSN：1863-2661
文摘
Serotoninergic innervation of the central nervous system is provided by hindbrain raphe nuclei (B1–B9). The extent to which each raphe subdivision has distinct topographic organization of their projections is still unclear. We provide a comprehensive description of the main targets of the rostral serotonin (5-HT) raphe subgroups (B5–B9) in the mouse brain. Adeno-associated viruses that conditionally express GFP under the control of the 5-HT transporter promoter were used to label small groups of 5-HT neurons in the dorsal (B7d), ventral (B7v), lateral (B7l), and caudal (B6) subcomponents of the dorsal raphe (DR) nucleus as well as in the rostral and caudal parts of the median raphe (MR) nucleus (B8 and B5, respectively), and in the supralemniscal (B9) cell group. We illustrate the distinctive and largely non-overlapping projection areas of these cell groups: for instance, DR (B7) projects to basal parts of the forebrain, such as the amygdala, whereas MR (B8) is the main 5-HT source to the hippocampus, septum, and mesopontine tegmental nuclei. Distinct subsets of B7 have preferential brain targets: B7v is the main source of 5-HT for the cortex and amygdala while B7d innervates the hypothalamus. We reveal for the first time the target areas of the B9 cell group, demonstrating projections to the caudate, prefrontal cortex, substantia nigra, locus coeruleus and to the raphe cell groups. The broad topographic organization of the different raphe subnuclei is likely to underlie the different functional roles in which 5-HT has been implicated in the brain. The present mapping study could serve as the basis for genetically driven specific targeting of the different subcomponents of the mouse raphe system. Keywords Genetic mouse models Anatomical tract-tracing GFP SERT Serotonin Tegmental nucleus Pearson correlation Olfactory bulb Habenula Prefrontal cortex Median raphe Dorsal raphe Hippocampus Amygdala Axon tracing AAV