Fabrication of macroporous protein-containing films through the reverse emulsions approach featuring β-cyclodextrin-conjugated PEG-PLGA copolymers

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• 作者：Ji-jie Wen ; Hong-guang Lu 吕宏光 ; De-e Liu ; Hui Gao 高辉
• 刊名：Chinese Journal of Polymer Science
• 出版年：2016
• 出版时间：June 2016
• 年：2016
• 卷：34
• 期：6
• 页码：730-738
• 全文大小：1,152 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Condensed Matter Physics
  Industrial Chemistry and Chemical Engineering
  Polymer Sciences
  Characterization and Evaluation of Materials
  
• 出版者：Chinese Chemical Society and Institute of Chemistry, CAS, co-published with Springer
• ISSN：1439-6203
• 卷排序：34

文摘

A series of β-cyclodextrin-conjugated 4-arm poly(ethylene glycol)-poly(lactide-co-glycolide) (4-arm PEG-PLGA) copolymers were synthesized by a ring-opening polymerization of D,L-lactide and glycolide using 4-arm PEG as initiator, and then conjugated with mono(6-ethylenediamine-6-deoxy)-β-cyclodextrin (CDen) or ethylenediamino-bridged bis-β-CD (BCDen). The chemical structures of copolymers were confirmed by 1H-NMR and FTIR spectroscopy. The β-CD-conjugated PEG-PLGA formed stable reverse micelles due to the formation of β-CD and bovine serum albumin (BSA) inclusion complexation, which could accommodate BSA in the organic solvent with improved encapsulation efficiency. Moreover, we demonstrated a one-step approach to construct macroporous protein-containing films using these reverse micelles. The films with ordered pore arrays were directly prepared from reverse micelles. Interestingly, the protein was totally located in the whole matrix except for the pores.Keywordsβ-CyclodextrinCopolymerReverse micellesOrdered porous structuresProteins