Expression of the Thellungiella halophila vacuolar H+-pyrophosphatase gene (TsVP) in cotton improves salinity tolerance and increases seed cotton yield in a saline field
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- 作者：Kewei Zhang ; Jiuling Song ; Xiugui Chen ; Tingting Yin ; Changbin Liu ; Kunpeng Li…
- 关键词：Cotton (Gossypium hirsutum) ; H+ ; PPase ; Salt tolerance ; Emergence rate ; Survival rate ; Seed cotton yield
- 刊名：Euphytica
- 出版年：2016
- 出版时间：September 2016
- 年：2016
- 卷：211
- 期：2
- 页码：231-244
- 全文大小：957 KB
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Physiology
Plant Sciences
Ecology - 出版者：Springer Netherlands
- ISSN：1573-5060
- 卷排序：211
文摘
The emergence and survival of cotton seedlings is crucial for cotton cultivation in saline fields. Limited seed cotton yield in saline fields is also a matter of concern for farmers. The expression of TsVP, a Thellungiella halophila gene encoding a vacuolar proton pumping pyrophosphatase, has been shown to improve the salinity tolerance of transgenic cotton under greenhouse conditions. However, the potential for TsVP to improve the seed cotton yield in saline fields has yet to be evaluated. In this study, the time of emergence, emergence rate, survival rate, carbon assimilation capacity during bud stage, seed cotton yield, and cotton fibre quality were determined in saline field trials using TsVP-overexpressing transgenic cotton and wild-type cotton plants. In a saline field, TsVP-overexpressing transgenic cotton emerged two days earlier than wild-type plants, and displayed greater emergence rate and survival rate. The seed cotton yield of TsVP-overexpressing transgenic cotton plants increased by an average of 14.81 % compared with that of wild-type plants, and cotton fibre quality also improved. In greenhouse conditions, TsVP-overexpressing plants also required a shorter time for 50 % emergence than wild-type plants when the NaCl concentration was greater than 100 mM. This research indicates that TsVP has the potential to improve seed cotton yield in saline fields.