Emodin Inhibits Inducible Nitric Oxide Synthase in a Rat Model of Craniocerebral Explosive Injury

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• 作者：Yuan Ma ; Xun Xia ; Jing-min Cheng ; Yong-qin Kuang ; Tao Yang…
• 关键词：Emodin ; Nitric oxide ; iNOS ; Traumatic brain injury
• 刊名：Neurochemical Research
• 出版年：2014
• 出版时间：September 2014
• 年：2014
• 卷：39
• 期：9
• 页码：1809-1816
• 全文大小：2,010 KB
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• 作者单位：Yuan Ma (1) (2)
  Xun Xia (1) (2)
  Jing-min Cheng (1) (2)
  Yong-qin Kuang (1)
  Tao Yang (1)
  Li-bin Yang (1)
  Kexia Fan (1)
  Jian-wen Gu (1) (3)
  
  1. Department of Neurosurgery, Chengdu Military General Hospital, People’s Liberation Army, Chengdu, China
  2. Graduate Management Team, Third Military Medical University, Chongqing, China
  3. Air Force Geneal Hospital of PLA, No.30 Fucheng Road, Haidian District, Beijing, China
  
• ISSN：1573-6903

文摘

To investigate the effects of emodin on blast-induced traumatic brain injury (bTBI) in a rat model. Eighty rats were randomly divided into 2 groups (the control group and the emodin-treated group; N?=?40 per group) and were used to establish the model of blast-induced traumatic brain injury. Ten minutes after the explosion, an isotonic saline solution (10?mg/kg) or emodin (10?mg/kg) were administered via an intraperitoneal injection to the control group and the emodin-treated group, respectively. At each time point (pre-explosion, 2, 6, 12, 24?h after explosion), 2 rats were used for the pathological assessment and 6 rats were used for the biochemical assessment. The concentration of nitric oxide (NO) and the expression and activity of inducible nitric oxide synthase (iNOS) were measured at each time point by spectrophotometry and western blot analysis. Light and electron microscopy showed that the brain damage in the emodin-treated group was less serious than that observed in the control group. The concentration of NO in the emodin-treated group was lower compared with the control group (p?p?iNOS. These findings suggest that emodin has a protective effect against bTBI. One possible mechanism may occur by inhibiting the expression and activity of iNOS and consequently decreasing the concentration of NO.