Isolation and characterization of microsatellite markers from Brown Eared-pheasant (Crossoptilon mantchuricum)

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• 作者：Yuming Fu ; Suping Li ; Dongming Li ; Minglu Wu and Yuefeng Wu
• 关键词：Crossoptilon mantchuricum ; Genetic marker ; Microsatellite ; Polymorphism
• 刊名：Conservation Genetics
• 出版年：2009
• 出版时间：December, 2009
• 年：2009
• 卷：10
• 期：6
• 页码：1993-1995
• 全文大小：156.3 KB

文摘

The Brown Eared-pheasant (Crossoptilon mantchuricum) is an endangered species endemic to China. Here we developed eight microsatellite loci using a modified biotin-capture method. In the analyses of 28 individuals sampled, the number of alleles per locus ranged from four to nine. The expected (H _E) and observed (H _O) heterozygosities were 0.466–0.825 and 0.619–0.847, respectively. Results that eight microsatellite loci were highly polymorphic indicated that these markers are sufficiently powerful to address such questions as genetic diversity and population genetic structure of C. mantchuricum.