Molecular cloning and characterization of seven class III peroxidases induced by overexpression of the agrobacterial rolB gene in Rubia cordifolia transgenic callus cultures

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• 作者：G. N. Veremeichik (1)
  Y. N. Shkryl (1) yn80@mail.ru
  V. P. Bulgakov (12)
  T. V. Avramenko (12)
  Y. N. Zhuravlev (1)
• 关键词：Peroxidase &#8211 ; Agrobacterium rhizogenes &#8211 ; Callus culture &#8211 ; RolB and rolC genes &#8211 ; Rubia cordifolia &#8211 ; Signal transduction
• 刊名：Plant Cell Reports
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：31
• 期：6
• 页码：1009-1019
• 全文大小：763.4 KB
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• 作者单位：1. Institute of Biology and Soil Science of the Far East Branch of Russian Academy of Sciences, 159 Stoletija Str., Vladivostok, 690022 Russia2. Far Eastern Federal University, 8 Sukhanova St., Vladivostok, 690950 Russia
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Cell Biology
  Plant Sciences
  Biotechnology
  Plant Biochemistry
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1432-203X

文摘

Here, seven new class III peroxidase genes of Rubia cordifolia L., RcPrx01–RcPrx07, were isolated and characterized. Expression of the Prx genes was studied in R. cordifolia aerial organs as well as in cells transformed with the rolB and rolC genes of Agrobacterium rhizogenes and cells transformed with the wild-type A. rhizogenes A4 strain. In rolC- and rolB-transformed cells, the rol genes were expressed under the control of the 35S promoter, whereas in A. rhizogenes A4-transformed cells the rol genes were expressed under the control of their native promoters. All studied peroxidase genes were greatly upregulated in rolB-overexpressing cells. In contrast, overexpression of the rolC gene and expression of the rol genes under the control of their native promoters had little effect on the abundance of peroxidase transcripts. In accordance with this observation, peroxidase activity was substantially increased in rolB cells and was slightly affected in other transformed cells. Our results indicate that rolB strictly affects the regulation of a set of seven R. cordifolia class III peroxidases.