Ectopic overexpression of a salt stress-induced pathogenesis-related class 10protein (PR10) gene from peanut (Arachis hypogaea L.) affords broad spectrum abiotic stress tolerance in transgenic tobacco
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- 作者：Shalu Jain (13)
Deepak Kumar (1)
Mukesh Jain (14)
Prerna Chaudhary (1)
Renu Deswal (2)
Neera Bhalla Sarin (1) neerasarin@rediffmail.com - 关键词：Abiotic stress – ; Abscisic acid – ; Arachis hypogaea – ; Jasmonic acid – ; PR proteins – ; Ribonuclease – ; Salicylic acid
- 刊名：Plant Cell, Tissue and Organ Culture
- 出版年：2012
- 出版时间：April 2012
- 年：2012
- 卷：109
- 期：1
- 页码：19-31
- 全文大小：748.3 KB
- 参考文献：1. Archambault A, Stromvik MV (2011) PR-10, defensing and cold dehydrin genes are among those over expressed in Oxytropis (Fabaceae) species adapted to the arctic. Funct Integr Genomics 11:497–505
2. Arnon DI (1949) Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. Plant Physiol 24:1–15
3. Bahramnejad B, Goodwin PH, Zhang J, Atnaseo C, Erickson LR (2010) A comparison of two class 10 pathogenesis-related genes from alfalfa to their activation by multiple stresses and stress-related signaling molecules. Plant Cell Rep 29:1235–1250
4. Blom N, Gammeltoft S, Brunak S (1999) Sequence and structure-based prediction of eukaryotic protein phosphorylation sites. J Mol Biol 294:1351–1362
5. Castro AJ, Carapito C, Zorn N, Magne C, Leize E, Dorsselaer AV (2005) Proteomic analysis of grapevine (Vitis vinifera L.) tissues subjected to herbicide stress. J Exp Bot 56:2783–2795
6. Chadha P, Das RH (2006) A pathogenesis related protein, AhPR10 from peanut: an insight of its mode of antifungal activity. Planta 225:213–222
7. Chhikara S, Chaudhury D, Dhankher OP, Jaiwal PK (2011) Combined expression of a barley class II chitinase and type I ribosomal inactivating protein in transgenic Brassica juncea provide protection against Alternaria brassicae. Plant Cell Tiss Organ Cult. doi:10.1007/s11240-011-0015-7
8. Cowan AK, Freeman M, Bj枚rkman PO, Nicander B, Sitbon F, Tillberg E (2005) Effects of senescence-induced alteration in cytokinin metabolism on source-sink relationships and ontogenic and stress-induced transitions in tobacco. Planta 221:801–814
9. Dubos C, Plomion C (2001) Drought differentially affects expression of a PR-10 protein, in needles of maritime pine (Pinus pinaster Ait.) seedlings. J Exp Bot 52:1143–1144
10. Fernandes H, Pasternak O, Bujacz G, Bujacz A, Sikorski MM, Jaskolski M (2008) Lupinus luteus pathogenesis-related protein as a reservoir for cytokinin. J Mol Biol 378:1040–1051
11. Flores T, Alape-Cir贸n A, Flores-D铆az M, Flores HE (2002) Ocatin, a novel tuber storage protein from the Andean tuber crop oca with antibacterial and antifungal activities. Plant Physiol 128:1291–1302
12. Gomez–Gomez L, Rubio-Moraga A, Ahrazem O (2011) Molecular cloning and characterization of a pathogenesis-related protein CsPR10 from Crocus sativus. Plant Biol 13:297–303
13. Guan B, Jiang G, Wang Y, Wang Z, Haxim Y, Bao Q (2010) Identification of differentially expressed transcripts involved in the salt-stress response of Salsola ferganica by suppression subtractive hybridization. Plant Cell Tiss Organ Cult 103:343–353
14. Gupta V, Willits MG, Glazebrook J (2000) Arabidopsis thaliana EDS4 contributes to salicylic acid (SA)-dependent expression of defense responses: evidence for inhibition of jasmonic acid signaling by SA. Mol Plant Microbe Interact 13:503–511
15. Haisel D, Vankova R, Synkova H, Posp铆šilov谩 J (2008) The impact of transzeatin O-glucosyltransferase gene over-expression in tobacco on pigment content and gas exchange. Biol Plant 52:49–58
16. Hashimoto M, Kisseleva L, Sawa S, Furukawa T, Komatsu S, Koshiba T (2004) A novel rice PR10 protein, RSOsPR10, specifically induced in roots by biotic and abiotic stresses, possibly via the jasmonic acid signaling pathway. Plant Cell Physiol 45:550–559
17. Hoffmann-Sommergruber K (2002) Pathogenesis-related (PR)-proteins identified as allergens. Biochem Soc Trans 30:930–935
18. Horsch RB, Fry JE, Hoffmann NL, Eichholtz D, Rogers SG, Fraley RT (1985) A simple and general method for transferring genes into plants. Science 227:1229–1230
19. Hwang EW, Kim KA, Park SC, Jeong MJ, Byun MO, Kwon HB (2005) Expression profiles of hot pepper (Capsicum annuum) genes under cold stress conditions. J Biosci 30:657–667
20. Jain M, Mathur G, Koul S, Sarin NB (2001) Ameliorative effects of proline on salt stress-induced lipid peroxidation in cell lines of groundnut (Arachis hypogaea L.). Plant Cell Rep 20:463–468
21. Jain S, Srivastava S, Sarin NB, Kav NNV (2006) Proteomics reveals elevated levels of PR10 proteins in saline-tolerant peanut (Arachis hypogaea) calli. Plant Physiol Biochem 44:253–259
22. Jwa NS, Kumar Agrawal G, Rakwal R, Park CH, Prasad Agrawal V (2001) Molecular cloning and characterization of a novel Jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves. Biochem Biophys Res Comm 286:973–983
23. Kav NNV, Srivastava S, Goonewardene L, Blade SF (2004) Proteome-level changes in the roots of Pisum sativum L. in response to salinity. Ann Appl Biol 145:217–230
24. Koistinen KM, Hassinen VH, Gynther PAM, Lehesranta SJ, Keinanen SI, Kokko HI, Oksanen EJ, Tervahauta AI, Auriola S, Karenlampi SO (2002) Birch PR-10c is induced by factors causing oxidative stress but appears not to confer tolerance to these agents. New Phytol 155:381–391
25. Lebel S, Schellenbaum P, Walter B, Mailot P (2010) Characterization of the Vitis vinifera PR10 multigene family. BMC Plant Biol 10:184–196
26. Liu JJ, Ekramoddoullah AKM (2006) The family 10 of plant pathogenesis-related proteins: their structure, regulation, and function in response to biotic and abiotic stresses. Physiol Mol Plant Pathol 68:3–13
27. Liu JJ, Ekramoddoullah AKM, Yu X (2003) Differential expression of multiple PR10 proteins in western white pine following wounding, fungal infection and cold-hardening. Physiol Planta 119:544–553
28. Liu X, Huang B, Lin J, Fei J, Chen Z, Pang Y, Sun X, Tang K (2006) A novel pathogenesis related protein (SsPR10) from Solanum surattense with ribonucleolytic and antimicrobial activity is stress- and pathogen inducible. J Plant Physiol 163:546–556
29. Livak KJ, Schmittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCt method. Methods 25:402–408
30. Ma Y, Szostkiewicz I, Korte A, Moes D, Yang Y, Christmann A, Grill E (2009) Regulators of PP2C phosphatase activity function as abscisic acid sensors. Science 324:1064–1068
31. Markovic-Housely Z, Degano M, Lamba D, Von Roepenack-Lahaye E, Clemens S, Susani M, Ferreira F, Scheiner O, Breitender H (2003) Crystal structure of a hypoallergenic isoforms of the major birch pollen allergen Bet v 1 and its likely biological function as a plant steroid carrier. J Mol Biol 325:123–133
32. Mittag D, Akkerdaas J, Ballmer-Weber BK, Vogel L, Wensing M, Becker WM, Koppelman SJ, Knulst AC, Helbling A, Hefle SL, Van Ree R, Vieths S (2004) Ara h 8, a Bet v 1-homologous allergen from peanut, is a major allergen in patients with combined birch pollen and peanut allergy. J Allergy Clin Immunol 114:1410–1417
33. Mogensen JE, Wimmer R, Larsen JN, Spangfort MD, Otzen DE (2002) The major birch allergen, Bet v 1, shows affinity for a broad spectrum of physiological ligands. J Biol Chem 277:23684–23692
34. Mur LAJ, Sturgess FJ, Farrell GG, Draper J (2004) The AoPR10 promoter and certain homologous PR10 genes report oxidative stress in Arabidopsis. Mol Plant Pathol 5:435–451
35. Muramoto Y, Watanable A, Nakamura T, Takabe T (1999) Enhanced expression of a nuclease gene in leaves of barley plants under salt stress. Gene 234:315–321
36. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Planta 15:473–497
37. Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight DNA. Nucleic Acids Res 8:4321–4325
38. Muthukrishnan S, Liang GH, Trick HN, Gill BS (2001) Pathogenesis-relates proteins and their genes in cereals. Plant Cell Tiss Organ Cult 64:93–114
39. Niki T, Mitsuhara I, Seo S, Ohtsubo N, Ohashi Y (1998) Antagonistic effect of salicylic acid and jasmonic acid on the expression of pathogenesis-related (PR) protein genes in wounded mature tobacco leaves. Plant Cell Physiol 39:500–507
40. Paakkonen E, Seppanen S, Holopainen T, Kokko H, Karenlampi S, Karenlampi L, Kangasjarvi J (1998) Induction of genes for the stress proteins PR-10 and PAL in relation to growth, visible injuries and stomatal conductance in birch (Betula pendula) clones exposed to ozone and/or drought. New Phytol 138:295–305
41. Park CJ, Kim KJ, Shin R, Park JM, Shin YC, Paek KH (2004) Pathogenesis-related protein 10 isolated from hot pepper functions as a ribonuclease in an antiviral pathway. Plant J 37:186–198
42. Pasquali G, Orbovic V, Grosser JW (2009) Transgenic grapefruit plants expressing the PAPETALA3-IPTgp gene exhibit altered expression of PR genes. Plant Cell Tiss Organ Cult 97:215–223
43. Posp铆šilov谩 J, Dodd IC (2005) Role of plant growth regulators in stomatal limitation to photosynthesis during water stress. In: Pessarakli M (ed) Handbook of photosynthesis, 2nd edn. Revised and expanded. Marcel Dekker, New York, pp 811–825
44. Radauer C, Lackner P, Breiteneder H (2008) The Bet v 1 fold: an ancient, versatile scaffold for binding of large, hydrophobic ligands. BMC Evol Biol 8:286
45. Rakwal R, Agrawal GK, Yonekura M (1999) Separation of proteins from stressed rice (Oryza sativa L.) leaf tissues by two-dimensional polyacrylamide gel electrophoresis: Induction of pathogenesis-related and cellular protectant proteins by jasmonic acid, UV irradiation and copper chloride. Electrophoresis 20:3472–3478
46. Rakwal R, Agrawal GK, Yonekura M (2001) Light dependent induction of OsPR10 in rice (Oriza sativa L.) seedlings by the global stress signaling molecule jasmonic acid and protein phosphatase 2A inhibitors. Plant Sci 161:479–489
47. Riecken S, Lindner B, Petersen A, Jappe U, Becker WM (2008) Purification and characterization of natural Ara h 8, the Bet v 1 homologous allergen from peanut, provides a novel isoform. Biol Chem 389:415–423
48. Rivero RM, Shulaev V, Blumwald E (2009) Cytokinin-dependent photorespiration and the protection of photosynthesis during water deficit. Plant Physiol 150:1530–1540
49. Salzman RA, Brady JA, Finlayson SA, Buchanan CD, Summer EJ, Sun F, Klein PE, Klein RR, Pratt LH, Cordonnier-PraH MM (2005) Transcriptional profiling of sorghum induced by methyl jasmonate, salicylic acid, and aminocyclopropane carboxylic acid reveals cooperative regulation and novel gene responses. Plant Physiol 138:352–368
50. Sambrook J, Fritsch EF, Maniatis T (1989) In molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory, Cold Spring Harbor
51. Saraste M, Sibbald PR, Wittinghofer A (1990) The P-loop: a common motif in ATP- and GTP- binding proteins. Trends Biochem Sci 15:430–434
52. Singh KB, Foley RC, O帽ate-S谩nchez L (2002) Transcription factors in plant defense and stress responses. Curr Opin Plant Biol 5:430–436
53. Srivastava S, Fristensky B, Kav NNV (2004) Constitutive expression of a PR10 protein enhances the germination of Brassica napus under saline conditions. Plant Cell Physiol 45:1320–1324
54. Srivastava S, Neil Emery RJ, Kurepin LV, Reid DM, Fristensky B, Kav NNV (2006) Pea PR10.1 is a ribonuclease and its transgenic expression elevates cytokinin levels. Plant Growth Regul 49:17–25
55. Srivastava S, Emery RJN, Rahman MH, Kav NNV (2007) A crucial role for cytokinins in pea ABR17-mediated enhanced germination and early seedling growth of Arabidopsis thaliana under saline and low-temperature stresses. J Plant Growth Regul 26:26–37
56. Subramanyam K, Sailaja KV, Subramanyam K, Rao DM, Lakshmidevi K (2011) Ectopic expression of an osmotin gene leads to enhanced salt tolerance in transgenic chilli pepper (Capsicum annum L.). Plant Cell Tiss Organ Cult 105:181–192
57. Sule A, Vanrobaeys F, Hajos J, Beeumen JV, Devreese B (2004) Proteomic analysis of small heat shock protein isoforms in barley shoots. Phytochemistry 65:1853–1863
58. Van Loon LC, Rep M, Pieterse CMJ (2006) Significance of inducible defense-related proteins in infected plants. Annu Rev Phytopathol 44:135–162
59. Wang CS, Huang JC, Hu JH (1999) Characterization of two subclasses of PR-10 transcripts in lily anthers and induction of their genes through separate signal transduction pathways. Plant Mol Biol 40:807–814
60. Wu F, Yan M, Li Y, Chang S, Song X, Zhou Z, Gong W (2003) cDNA cloning, expression, and mutagenesis of a PR-10 protein SPE-16 from the seeds of Pachyrrhizus erosus. Biochem Biophys Res Commun 312:761–766
61. Xie YR, Chen ZY, Brown RL, Bhatnagar D (2010) Expression and functional characterization of two pathogenesis-related protein 10 genes from Zea mays. J Plant Physiol 167:121–130
62. Xiong L, Yang Y (2003) Disease resistance and abiotic stress tolerance in rice are inversely modulated by an abscisic acid-inducible mitogen-activated protein kinase. Plant Cell 15:745–759 - 作者单位：1. Plant Developmental Biology and Transformation Lab, School of Life Sciences, Jawaharlal Nehru University, New Delhi, 110067 India2. Department of Botany, University of Delhi, Delhi, 110007 India3. Department of Plant Sciences, North Dakota State University, Fargo, ND 58102, USA4. Agronomy Department, University of Florida, Gainesville, FL 32610, USA
- 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Plant Sciences
Plant Physiology - 出版者：Springer Netherlands
- ISSN：1573-5044
文摘
Pathogenesis-related proteins are induced in plants in response to stress, pathogen attack or abiotic stimuli, thus playing a cardinal role in plant defense system. A cDNA containing the full-length ORF, AhSIPR10 (474 bp, GenBank acc. no. DQ813661), encoding a novel Salinity-Induced PR class 10 protein was isolated from callus cell lines of peanut (Arachis hypogaea). Real-time quantitative reverse transcription PCR (qRT–PCR) data showed rapid upregulation of AhSIPR10 transcription in peanut callus cultures across salinity, heavy metal, cold and mannitol-induced drought stress environments. Likewise, AhSIPR10 expression was also responsive towards defense/stress signaling molecules salicylic acid (SA), methyl jasmonate, abscisic acid (ABA) and H2O2 treatments. Methyl jasmonate or ABA-induced AhSIPR10 expression was, however, antagonized by SA treatment. A functional role of AhSIPR10 in alleviation of abiotic stress tolerance was further validated through its over-expression in tobacco. Analysis of T1 transgenic tobacco plants overexpressing AhSIPR10 gene showed enhanced tolerance to salt, heavy metal and drought stress through leaf disc senescence, chlorophyll content, seed set and germination assays, thus corroborating a role of salt inducible-PR10 protein in mitigation of abiotic stress-induced damage. Transgenic tobacco lines overexpressing AhSIPR10 displayed adequate photosynthetic CO2 assimilation rates under salt, heavy metal and drought stress environments.