Somatic embryogenesis from ovaries of sweet orange cv. Tobias

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• 作者：Jean C. Cardoso (1) jeancardosoctv@gmail.com
  Adriana P. Martinelli (1)
  Rodrigo R. Latado (2)
• 关键词：Citrus sinensis &#8211 ; Pistil culture &#8211 ; Callus &#8211 ; Culture medium &#8211 ; Plantlet regeneration
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2012
• 出版时间：April 2012
• 年：2012
• 卷：109
• 期：1
• 页码：171-177
• 全文大小：1.1 MB
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• 作者单位：1. Centro de Energia Nuclear na Agricultura, Universidade de S茫o Paulo, Av. Centen谩rio, 303, Piracicaba, SP 13416-000, Brazil2. Centro APTA Citros Sylvio Moreira, IAC, Rod Anhanguera, km 158, CP 04, Cordeir贸polis, SP CEP 13490-970, Brazil
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

Callogenesis, somatic embryogenesis, and regeneration were obtained from tissues of unfertilized ovaries of sweet orange (Citrus sinensis Osbeck.) cv. Tobias. The influence of two modified basal media, woody plant medium (WPM) and N6 medium, to induce callus formation from pistils was determined. Overall, high frequencies of callogenesis were observed when either medium was used. However, initial culture of explants in WPM medium followed by transfer of callus to N6 medium resulted in higher frequency of callus induction (of 2.30 callus per explant that were larger than 0.5 cm in size), and of subsequent development of embryogenic callus (10%). A total of 125 somatic embryos were obtained. After 6 months of culture, 72% of somatic embryos germinated into plantlets. These plantlets were subsequently micrografted in vitro, and then acclimatized. Ploidy of these plants were determined using flow cytometry and TRAPS molecular markers were used to confirm their maternal origin.