Enhanced regeneration of haploid plantlets from microspores of Brassica napus L. using bleomycin, PCIB, and phytohormones

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• 作者：Behzad Ahmadi (1)
  Khoshnood Alizadeh (2)
  Jaime A. Teixeira da Silva (3) jaimetex@yahoo.com
• 关键词：Anti ; auxin &#8211 ; Bleomycin &#8211 ; Phytohormone &#8211 ; Rapeseed &#8211 ; Secondary embryogenesis
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2012
• 出版时间：June 2012
• 年：2012
• 卷：109
• 期：3
• 页码：525-533
• 全文大小：358.5 KB
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• 作者单位：1. Department of Agronomy and Plant Breeding, Faculty of Agriculture, Urmia University, Urmia, Iran2. Mahabad Branch, Islamic Azad University, Mahabad, Iran3. Faculty of Agriculture and Graduate School of Agriculture, Kagawa University, Miki cho, Kita gun, Ikenobe, 761-0795 Japan
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  Plant Physiology
  
• 出版者：Springer Netherlands
• ISSN：1573-5044

文摘

The effects of three periods of incubation (10, 20 and 30 min) at different levels of bleomycin (0, 0.1, 0.2, 0.3, 0.4 and 0.5 μg ml−1), as well as three periods of exposure (12, 24 and 48 h) to different levels of the anti-auxin p-chlorophenoxyisobutyric acid (PCIB), including 1, 2, 3, 4 and 5 mg l−1, on microspore embryogenesis of rapeseed cv. ‘Amica’ were investigated. Microspore embryogenesis was significantly enhanced following 20 min treatment with 0.2 μg ml−1 bleomycin compared with untreated cultures. Highest embryo yield (163 embryos Petri dish−1) was observed with 24 h treatment of 4 mg l−1 PCIB. The highest percentage of secondary embryogenesis was observed on B₅ medium containing 0.15 mg l−1 of gibberellic acid (GA₃) and 0.2 mg l−1 6-benzyladenine (BA) in 4–6 mm microspore-derived embryos (MDEs). Most callus formed on B₅ medium containing 0.15 mg l−1 GA₃, 0.1 mg l−1 BA and 0.1 mg l−1 indole-3-acetic acid (IAA) when 4–6 mm embryos were used. Regeneration was highest on B₅ medium containing 0.05 mg l−1 GA₃ or 0.1 mg l−1 BA and 0.2 mg l−1 IAA with 2–4 mm embryos. Microspore embryogenesis and plant regeneration could be improved by both bleomycin and PCIB when the appropriate MDE length and phytohormone level were selected.