An Adenoviral Vector Encoding Full-Length Dectin-1 Promotes Aspergillus-Induced Innate Immune Response in Macrophages

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• 作者：Di Xia ; Wen-Kui Sun ; Ming-Ming Tan ; Yuan Ding ; Zhi-Cheng Liu ; Pei Li ; Qian Qian…
• 关键词：Dectin ; 1 ; Aspergillus ; Adenoviral vector ; Innate immune response ; Macrophages
• 刊名：Lung
• 出版年：2015
• 出版时间：August 2015
• 年：2015
• 卷：193
• 期：4
• 页码：549-557
• 全文大小：1,259 KB
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• 作者单位：Di Xia (1)
  Wen-Kui Sun (1)
  Ming-Ming Tan (1)
  Yuan Ding (1)
  Zhi-Cheng Liu (2)
  Pei Li (1)
  Qian Qian (3)
  Xin Su (1)
  Yi Shi (1)
  
  1. Department of Respiratory and Critical Care Medicine, Jinling Hospital, Medical School of Nanjing University, Nanjing, People’s Republic of China
  2. Southern Medical University, Guangdong, People’s Republic of China
  3. Nanjing Chest Hospital, Nanjing, People’s Republic of China
  
• 刊物类别：Medicine
• 刊物主题：Medicine & Public Health
  Pneumology and Respiratory System
  
• 出版者：Springer New York
• ISSN：1432-1750

文摘

Introduction The incidence of invasive pulmonary aspergillosis (IPA) has increased significantly over the last two decades. Alveolar macrophages (AMs) represent the first line of pulmonary host response to Aspergillus conidia. Recognition of conidia by AMs involves Dectin-1 (CLEC7A), which is a conserved structure to combine β-glucans. The deficiency of Dectin-1 results in impaired fungal killing and uncontrolled growth of Aspergillus fumigatus. Thus, we hypothesized that high expression of Dectin-1 would enhance the host recognition and fungal killing. Methods We set out to develop an adenoviral vector encoding full-length Dectin-1 (Ad-Dectin-1-EGFP) and then transfect it to MH-S cells. Transfect cell model was verified by using real-time RT-PCR, Western blot, flow cytometric, and confocal microscopic assays. And also, the function of Dectin-1 was explored by measuring cytokine release and killing ability during the course of A. fumigatus infection. Results We constructed a recombinant adenovirus which could upregulate the expression of Dectin-1 and verified that Dectin-1 was expressed on cell membrane. The function of Dectin-1 was also demonstrated by its ability in promoting the production of cytokines and increasing the killing ability during the course of A. fumigatus infection. Conclusions An adenoviral vector was successfully applied to the production of a recombinant adenovirus encoding full-length Dectin-1, and also, its function in Aspergillus-induced innate immune response was demonstrated.