Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production
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- 作者：Fangmin Bai ; Lu Dai ; Jiying Fan…
- 关键词：(3R) ; acetoin ; (2R ; 3R) ; 2 ; 3 ; butanediol ; Meso ; 2 ; 3 ; butanediol dehydrogenase ; (2R ; 3R) ; 2 ; 3 ; butanediol dehydrogenase ; Serratia marcescens
- 刊名：Journal of Industrial Microbiology and Biotechnology
- 出版年：2015
- 出版时间：May 2015
- 年：2015
- 卷：42
- 期：5
- 页码：779-786
- 全文大小：506 KB
- 参考文献：1. Borim, K, Lee, S, Park, J, Lu, M, Oh, M, Kim, Y, Lee, J (2012) Enhanced 2,3-butanediol production in recombinant Klebsiella pneumoniae via overexpression of synthesis-related genes. J Microbiol Biotechnol 22: pp. 1258-1263 CrossRef
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- 刊物主题：Chemistry
Biotechnology
Genetic Engineering
Biochemistry
Bioinformatics
Microbiology
Microbial Genetics and Genomics - 出版者：Springer Berlin / Heidelberg
- ISSN：1476-5535
文摘
(3R)-Acetoin and (2R,3R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3R)-acetoin and (2R,3R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2S,3S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2S,3S)-2,3-butanediol and result in a large quantity of (3R)-acetoin accumulation. Furthermore, a (2R,3R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2R,3R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol and lead to (2R,3R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2R,3R)-2,3-butanediol dehydrogenase expression strain could produce 89.81?g/l (2R,3R)-2,3-butanediol with a productivity of 1.91?g/l/h at 48?h. These results provided potential applications for (3R)-acetoin and (2R,3R)-2,3-butanediol production.