Characterization, drug release profile and cytotoxicity of Dentatin-Hydroxypropyl-β-Cyclodextrin complex

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• 作者：Al-Abboodi Shakir Ashwaq ; Abdullah Rasedee…
• 关键词：Dentatin ; Hydroxypropyl ; β ; cyclodextrin ; Physical properties ; Solubility ; Drug release
• 刊名：Journal of Inclusion Phenomena and Macrocyclic Chemistry
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：87
• 期：1-2
• 页码：167-178
• 全文大小：
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Organic Chemistry; Food Science; Crystallography and Scattering Methods;
• 出版者：Springer Netherlands
• ISSN：1573-1111
• 卷排序：87

文摘

This current work has been conducted mainly to increase solubility and drug release properties for high hydrophobic Dentatin (DEN) by incorporation it into Hydroxypropyl-β-Cyclodextrin (HPβCD) cavity. To confirm that inclusion be succeeded, the produced complex were installed onto different machines. The latter includes: Fourier transform infrared spectroscopy (FT-IR), X-ray diffractometry (XRD), differential scanning calorimetry (DSC), and field emission-scanning electron microscopy (FE-SEM). The hydrodynamic diameter and zeta potential of DEN-HPβCD complex were 2.025 ± 0.39 nm and −33.6 mV, respectively. Ultra-violet spectroscopy was employed to further confirmation of complexation process as well as to determine drug release profile. The result showed an initial burst release (19.9% within first two minutes) and then a continuous release for an extended period of 41 h (100%). The solubility of DEN was enhanced by >300 fold following complexation when a compared to DEN alone. Moreover, MTT finding showed that this complexation did not reduce cytotoxicity of DEN after applying on prostate cancer (LNCaP), human adenocarcinoma breast cancer (MDA-MB-231) and human gastric adenocarcinoma cell line (HDT). However, further investigations are required to validate efficacy of our produced inclusion using molecular analysis and in vivo studies.