Gene Expression Profile of NF-κB, Nrf2, Glycolytic, and p53 Pathways During the SH-SY5Y Neuronal Differentiation Mediated by Retinoic Acid
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- 作者：Matheus Augusto de Bittencourt Pasquali ; Vitor Miranda de Ramos…
- 关键词：Neuronal differentiation ; Neuroblastoma ; Retinoic acid ; SH ; SY5Y cells ; Oxidative stress
- 刊名：Molecular Neurobiology
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：53
- 期：1
- 页码：423-435
- 全文大小：1,638 KB
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Vitor Miranda de Ramos (1)
Ricardo D′Oliveira Albanus (1)
Alice Kunzler (1)
Luis Henrinque Trentin de Souza (1)
Rodrigo Juliani Siqueira Dalmolin (2)
Daniel Pens Gelain (1)
Leila Ribeiro (3)
Luigi Carro (3)
José Cláudio Fonseca Moreira (1)
1. Departamento de Bioquímica, Centro de Estudos em Estresse Oxidativo, Universidade Federal do Rio Grande do Sul—UFRGS, Rua Ramiro Barcelos 2600, Cep 90035-003, Porto Alegre, Rio Grande do Sul, Brazil
2. Departamento de Bioquímica, Instituto de Medicina Tropical, Universidade Federal do Rio Grande do Norte—UFRN, Avenida Senador Salgado Filho 3000, Cep 59078-900, Natal, Rio Grande do Norte, Brazil
3. Departamento de Informática Aplicada, Universidade Federal do Rio Grande do Sul—UFRGS, Rua Bento Gonçalves 9500, Cep 91501-970, Porto Alegre, Rio Grande do Sul, Brazil - 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
- 出版者：Springer US
- ISSN：1559-1182
文摘
SH-SY5Y cells, a neuroblastoma cell line that is a well-established model system to study the initial phases of neuronal differentiation, have been used in studies to elucidate the mechanisms of neuronal differentiation. In the present study, we investigated alterations of gene expression in SH-SY5Y cells during neuronal differentiation mediated by retinoic acid (RA) treatment. We evaluated important pathways involving nuclear factor kappa B (NF-κB), nuclear E2-related factor 2 (Nrf2), glycolytic, and p53 during neuronal differentiation. We also investigated the involvement of reactive oxygen species (ROS) in modulating the gene expression profile of those pathways by antioxidant co-treatment with Trolox®, a hydrophilic analogue of α-tocopherol. We found that RA treatment increases levels of gene expression of NF-κB, glycolytic, and antioxidant pathway genes during neuronal differentiation of SH-SY5Y cells. We also found that ROS production induced by RA treatment in SH-SY5Y cells is involved in gene expression profile alterations, chiefly in NF-κB, and glycolytic pathways. Antioxidant co-treatment with Trolox® reversed the effects mediated by RA NF-κB, and glycolytic pathways gene expression. Interestingly, co-treatment with Trolox® did not reverse the effects in antioxidant gene expression mediated by RA in SH-SY5Y. To confirm neuronal differentiation, we quantified endogenous levels of tyrosine hydroxylase, a recognized marker of neuronal differentiation. Our data suggest that during neuronal differentiation mediated by RA, changes in profile gene expression of important pathways occur. These alterations are in part mediated by ROS production. Therefore, our results reinforce the importance in understanding the mechanism by which RA induces neuronal differentiation in SH-SY5Y cells, principally due this model being commonly used as a neuronal cell model in studies of neuronal pathologies.