Characterization of antifungal activity of Paenibacillus ehimensis KWN38 against soilborne phytopathogenic fungi belonging to various taxonomic groups

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• 作者：Kyaw Wai Naing (1)
  Muhammad Anees (2)
  Sang Jun Kim (3)
  Yi Nam (4)
  Young Cheol Kim (1)
  Kil Yong Kim (1)
  
• 关键词：Antagonism ; Chitinase ; 尾 ; 1 ; 3 ; glucanases ; Hyphal inhibition ; Paenibacillus ehimensis ; Soilborne fungi
• 刊名：Annals of Microbiology
• 出版年：2014
• 出版时间：March 2014
• 年：2014
• 卷：64
• 期：1
• 页码：55-63
• 全文大小：633 KB
• 作者单位：Kyaw Wai Naing (1)
  Muhammad Anees (2)
  Sang Jun Kim (3)
  Yi Nam (4)
  Young Cheol Kim (1)
  Kil Yong Kim (1)
  
  1. Institute of Environmentally-Friendly Agriculture, College of Agriculture and Life Sciences, Chonnam National University, Gwangju, 500-757, Korea
  2. Department of Microbiology, Kohat University of Science and Technology, Kohat, Pakistan
  3. Corporation of Nature and People, Okbongri 1759-12, Okseomeon, Gunsan, 573-952, Korea
  4. National Agricultural Cooperative Federation, Ansung, Ansung Training Institute, Gongdoeup, Sinduri, 336-1, Korea
  
• ISSN：1869-2044

文摘

Soilborne fungal phytopathogens cause significant losses in many economically important crops and vegetables. The only way to control these devastating pathogens is by using higher doses of fungicides which not only increase the cost of production but also cause significant damage to the environment. Therefore alternate control measures are always looked for. In the present study, an antagonistic strain was isolated from the soil of the pepper fields around the seashore of Jellanamdo, South Korea and identified as Paenibacillus ehimensis KWN38 based on 16S rRNA sequencing. The strain showed high antifungal activity against six tested fungal pathogens belonging to various taxonomic groups on dual culture plates. Furthermore, the strain produced volatile antimicrobial compounds which had strong fungal growth inhibitory effect. The strain also showed high chitinase, cellulase, glucanase and protease activities. The hyphal morphologies of Rhizoctonia solani AG-1 (IA), Fusarium oxysporum f.sp. lycopersici and Phytophthora capsici were significantly destroyed by the crude enzymes and butanol extract from the culture supernatant and the affected hyphae showed abnormal bending, tip curling, and irregular branching. Hence, Paenibacillus ehimensis KWN38 is considered as a potential biocontrol agent of the soil-borne fungi causing plant diseases which is an important perspective of the present study.