Manufacturing and structural analysis of antimicrobial kefiran/polyethylene oxide nanofibers for food packaging

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• 作者：Anahita Jenab ; Rasoul Roghanian ; Giti Emtiazi ; Kamran Ghaedi
• 关键词：Kefiran ; ATR ; FTIR ; Electrospun nanofiber ; Antimicrobial activity ; Food packaging
• 刊名：Iranian Polymer Journal
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：26
• 期：1
• 页码：31-39
• 全文大小：
• 刊物主题：Polymer Sciences; Ceramics, Glass, Composites, Natural Materials;
• 出版者：Springer Berlin Heidelberg
• ISSN：1735-5265
• 卷排序：26

文摘

Kefiran, a branched glucogalactan, is a useful microbial polysaccharide produced by lactic acid bacteria. The aim of this study was to evaluate the antimicrobial activity of kefiran nanofibers as a biocontrol agent for food packaging as well as food preservation. Thus, kefiran/polyethylene oxide (PEO) nanofibers were fabricated using the electrospinning method. Kefiran was produced from raw milk with 0.5% fat and 10 g of kefir grain and was separated from it by ethanol. Structural analysis of kefiran was detected by means of nuclear magnetic resonance and Fourier transforms infrared spectroscopy (FTIR). Antimicrobial properties of kefiran were assayed against Rhizoctonia, Pseudomonas sp. (isolated from soil at Isfahan University) and Staphylococcus aureus (S. aureus). Electrospun kefiran/PEO nanofibers were characterized by scanning electron microscopy, optical microscopy and ATR-FTIR techniques. Hydrophilicity and in vitro biodegradation of the kefiran/PEO nanofibers were investigated, as well. The results showed that the mean diameter of the nanofibers was 607.5 nm. The contact angle measurement result was 51.5° ± 0.71 with normal hydrophilicity. First of all, antimicrobial properties of kefiran were confirmed against different types of microorganisms. Moreover, the result obtained in this study showed that kefiran/PEO nanofibers with oxidizing functional groups on them have antimicrobial activity against S. aureus. Biodegradation of kefiran/PEO nanofibers was also confirmed by FTIR.