A novel neutral, halophile Stachybotrys microspora-based endoglucanase active impact on β-glucan

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• 作者：Ines Benhmad ; Manel Boudabbous ; Asma Yaîch…
• 关键词：Stachybotrys microspora ; Halophile ; Neutral endoglucanase ; Organic solvents ; Barley ; β ; glucan
• 刊名：Bioprocess and Biosystems Engineering
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：39
• 期：4
• 页码：685-693
• 全文大小：1,145 KB
• 参考文献：1.Zhang G, Li S, Xue Y, Mao L, Ma Y (2012) Effects of salts on activity of halophilic cellulase with glucomannanase activity isolated from alkaliphilic and halophilic Bacillus sp, BG-CS10. Extremophiles 16:35–43CrossRef
  2.Margesin R, Schinner F (2001) Potential of halotolerant and halophilic microorganisms for biotechnology. Extremophiles 5:73–83CrossRef
  3.Doukyu N, Ogino H (2010) Organic solvent tolerant enzymes. Biochem Eng J 48:270–282CrossRef
  4.Miettinen-Oinonen A, Londesborough J, Joutsjoki V, Lantto R, Vehmaanper J, Biotecd PL (2004) Three cellulases from Melanocarpus albomyces for textile treatment at neutral pH. Enzyme Microb Technol 34:332–341CrossRef
  5.Karmakar M, Ray RR (2011) Current trends in research and application of microbial cellulases. Res J Microbiol 6:41–53CrossRef
  6.Lee SM, Koo YM (2001) Pilot-scale production of cellulose using Trichoderma reesei Rut C-30 in fed-batch mode. J Microbiol Biotechnol 11:229–233
  7.Kim KH, Brown KM, Harris PV, Langston JA, Cherry JR (2007) A proteomic strategy to discover β-glucosidases from Aspergillus fumigatus with two-dimensional page in-gel activity assay and tandem mass spectrometry. J Proteome Res 6:4749–4757CrossRef
  8.Koga J, Baba Y, Shimonaka A, Nishimura T, Hanamura S, Kono T (2008) Purification and characterization of a new family 45 endoglucanase, STCE1, from Staphylotrichum coccosporum and its overproduction in Humicola insolens. Appl Environ Microbiol 74:4210–4217CrossRef
  9.Gupta V, Prasanna R, Chaudhary V, Nain L (2012) Biochemical, structural and functional characterization of two novel antifungal endoglucanases from Anabaena laxa. Biocatal Agric Biotechnol 1:338–347
  10.Amouri B, Gargouri A (2006) Characterization of a novel β-glucosidase from a Stachybotrys strain. Biochem Eng J 32:191–197CrossRef
  11.Ben Hmad I, Abdeljalil S, Saibi W, Amouri B, Gargouri A (2014) Medium initial pH and carbon source stimulate differential alkaline cellulase time course production in Stachybotrys microspora. Appl Biochem Biotechnol 172:2640–2649CrossRef
  12.Mandels M, Weber J (1969) The production of the cellulases. Adv Chem Ser 95:391CrossRef
  13.Miller GL (1959) Use of dinitrosalicylic acid reagent for determination of reducing sugar. Anal Chem 31:426–428CrossRef
  14.Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Chem 72:248–254
  15.Laemmli UK, Favre M (1973) Maturation of the head of bacteriophage T4. I. DNA packaging events. J Mol Biol 80:575–592CrossRef
  16.Saibi W, Amouri B, Gargouri A (2007) Purification and biochemical characterization of a transglucosilating β-glucosidase of Stachybotrys strain. Appl Microbiol Biotechnol 77:293–300CrossRef
  17.Saibi W, Gargouri A (2011) Purification and biochemical characterization of an atypical β-glucosidase from Stachybotrys microspora. J Mol Catal B Enzym 72:107–115CrossRef
  18.Belghith H, Ellouz-Chaabouni S, Gargouri A (2001) Biostoning of denims by Penicillium occitanis (Pol6). J Biotechnol 89:257–262CrossRef
  19.Kaur J, Chadha BS, Kumar BA, Saini HS (2007) Purification and characterization of two endoglucanases from Melanocarpus sp. MTCC 3922. Bioresour Technol 98:74–81CrossRef
  20.Wood TM, Mc Crae SI (1978) The Cellulase of Trichoderma koningii purification and properties of some endoglucanase components with special reference to their action on cellulose when acting alone and in synergism with the cellobiohydrolase. Biochem J 171:61–72CrossRef
  21.Trivedi N, Vishal G, Manoj K, Puja K, Reddy CRK, Jha B (2011) An alkali-halotolerant cellulase from Bacillus flexus isolated from green seaweed Ulva lactuca. Carbohyd Polym 83:891–897CrossRef
  22.Buckeridge MS, Goldman GH (2011) Routes to cellulosic ethanol. Springer, New YorkCrossRef
  23.Ch Li D, Lu M, Li YL, Lu J (2003) Purification and characterization of an endocellulase from the thermophilic fungus Chaetomium thermophilum CT2. Enzyme Microb Technol 33:932–937CrossRef
  24.Karnchanatat A, Petsom A, Sangvanich P, Piapukiew J, Whalley AJS, Rey-Nolds CD, Gadd GM, Sihanonth P (2008) A novel thermostable endoglucanase from the wood-decaying fungus Daldinia eschscholzii (Ehrenb.:Fr.) Rehm. Enzyme Microb Technol 42:404–413CrossRef
  25.Jakubowski H (2002) Enzyme catalysis in organic solvents in UCDavis-Biowiki. http://​biowiki.​ucdavis.​edu/​Wikitexts/​Biochemistry_​322:​_​Jakubowski
  26.Zaks A, Klibanov AM (1985) Biochemistry Enzyme-catalyzed processes in organic solvents. Proc Natl Acad Sci USA 82:3192–3196CrossRef
  
• 作者单位：Ines Benhmad (1)
  Manel Boudabbous (1)
  Asma Yaîch (1)
  Maryem Rebai (1)
  Ali Gargouri (1)
  
  1. Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax (CBS), University of Sfax, B.P 1177, 3018, Sfax, Tunisia
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Biotechnology
  Industrial Chemistry and Chemical Engineering
  Industrial and Production Engineering
  Waste Management and Waste Technology
  Waste Water Technology, Water Pollution Control, Water Management and Aquatic Pollution
  Food Science
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1615-7605

文摘

The production of cellulases from Stachybotrys microspora strain (A19) has been improved by fed-batch fermentation on Avicel cellulose 10 mg/ml. An endoglucanase EG2 was purified to homogeneity. This cellulase has a molecular mass estimated to 50 kDa when analyzed by a denaturant gel electrophoresis. It exhibited an optimal activity at 50 °C, pH 7.0 and 0.85 M NaCl. Specifically, these results show the thermo-active, alkali-tolerant and halo-tolerant properties of EG2. In addition, this endoglucanase showed its highest activity on barley-β-glucan, compared to the CMC. Moreover, it was less active on Avicel cellulose. Furthermore, the EG2 activity was stimulated in the presence of EDTA, urea and β-mercaptoethanol whereas it was reduced in the presence of SDS. This cellulase was highly stable in the presence of organic solvents such as acetone and n-hexane. TLC showed that the main hydrolysis products from EG2 were cellobiose and glucose. This fungal endoglucanase could be potentially important in the conversion of grass-derived biomass into fermentable sugars.