Diversity of species and antibiotic resistance among fecal enterococci from wild birds in Tunisia. Detection of vanA-containing Enterococcus faecium isolates

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• 作者：Naouel Klibi ; Insaf Ben Amor ; Marwa Rahmouni…
• 关键词：Enterococci ; Tunisia ; Birds ; Wild ; VanA ; E. faecium
• 刊名：European Journal of Wildlife Research
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：61
• 期：2
• 页码：319-323
• 全文大小：165 KB
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• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Zoology
  Animal Ecology
  
• 出版者：Springer Berlin / Heidelberg
• ISSN：1439-0574

文摘

Avifauna could be vectors of resistant bacteria including enterococci, but scarce information does exist about prevalence of antibiotic-resistant bacteria on bird populations, especially in the African Continent. Fecal samples of 111 birds (66 residents, 45 migratory) were taken in Tunisia in 2012. They were inoculated in Slanetz-Bartley agar supplemented or not with 8?μg/mL vancomycin (SB-Van and SB plates, respectively) for enterococci and vancomycin-resistant-enterococci (VRE) recovery. Enterococci were recovered in SB-plates in 73 of 111 samples (65.8?%) and one enterococci/positive sample was further studied: 60 Enterococcus faecium, 6 Enterococcus faecalis, 3 Enterococcus casseliflavus, 3 Enterococcus gallinarum, and 1 Enterococcus durans. Percentages of antimicrobial resistance identified were as follows: erythromycin (53.4?%), ciprofloxacin (41.1?%), tetracycline (19.2?%), pristanamycin (17.8?%), kanamycin, gentamicin or streptomycin (6.8-6.4?%), and ampicillin (0?%). Most of erythromycin-resistant and tetracycline-resistant enterococci carried the erm(B) and tet(M)+/?em class="a-plus-plus">tet(L) genes, respectively. Most of high-level gentamicin or kanamycin resistant isolates carried the aac(6--Ie-aph(2--Ia or aph(3--IIIa genes, respectively, and 40?% of high-level streptomycin-resistant isolates the ant(6)-Ia gene. The vat(D) gene was detected in 46?% of pristinamycin-resistant enterococci and the cat(A) gene in 40?% of chloramphenicol-resistant enterococci. The gel(E), ace, or hyl genes were detected in 8-2?% of enterococci. VRE was detected in four of the birds, when SB-Van plates were used. These isolates were identified as E. faecium and carried the vanA gene, as well as the erm(B), tetM, aph (3--IIIa, and vatD genes, but not the hyl and esp virulence genes. Wild birds are occasional carriers of vanA-containing enterococci that could have implications in public health.