Immunoassay for troponin I using a glassy carbon electrode modified with a hybrid film consisting of graphene and multiwalled carbon nanotubes and decorated with platinum nanoparticles

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• 作者：Shobhita Singal ; Avanish K Srivastava ; Bhasker Gahtori ; Rajesh
• 关键词：High resolution transmission electron microscopy ; Cyclic voltammetry ; Bioelectrode ; Graphene ; Electrochemical impedance spectroscopy ; Biomarker ; Myocardial infraction
• 刊名：Microchimica Acta
• 出版年：2016
• 出版时间：April 2016
• 年：2016
• 卷：183
• 期：4
• 页码：1375-1384
• 全文大小：677 KB
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• 作者单位：Shobhita Singal (1) (2)
  Avanish K Srivastava (1)
  Bhasker Gahtori (1)
  Rajesh (1) (2)
  
  1. CSIR-National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi, -110012, India
  2. Academy of Scientific and Innovative research (AcSIR), CSIR-National Physical Laboratory, Dr. K.S. Krishnan Road, New Delhi, -110012, India
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

This article describes a bioelectrode for the determination of human cardiac troponin-I (cTnI). A glassy carbon electrode was coated with a hybrid film of graphene and multiwalled carbon nanotube (G-MWCNT) and modified with platinum nanoparticles (Pt NPs) that were capped with mercaptopropionic acid. The PtNPs were anchored on the G-MWCNT hybrid film via the cross-linker 1-pyrenemethylamine and subsequently functionalized with antibody against troponin (anti-cTnI). The bioelectrode was characterized by transmission electron microscopy, scanning electron microscopy, cyclic voltammetry, and electrochemical impedance spectroscopy. The performance of the immunoelectrode was investigated by electrochemical impedance spectroscopy, and response was fit to Randle’s equivalent circuit model. The charge transfer resistance (R_et) at a.c. frequencies of <1 Hz is found to be a viable sensing parameter. The dissociation constant of the immunoreaction between surface immobilized anti-cTnI and the analyte cTnI is 0.29 nM (with a Hill coefficient of 0.23), this indicating a negative cooperativity and high binding affinity of cTnI for anti-cTnI on the electrode surface. The EIS response is linear in the 1.0 pg mL−1 to 10 ng mL−1 concentration range, and the R_et sensitivity is 145.5 Ω cm2 per decade.