Development of 240 novel EST-SSRs in Eucalyptus L’Hérit

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• 作者：Changpin Zhou (1) (2)
  Xudong He (3)
  Fagen Li (2)
  Qijie Weng (2)
  Xiaoli Yu (2)
  Yu Wang (2)
  Mei Li (2)
  Jisen Shi (3)
  Siming Gan (1) (2)
  
• 关键词：Eucalyptus ; Expressed sequence tag (EST) ; Simple sequence repeat (SSR) ; Polymorphism ; Transferability
• 刊名：Molecular Breeding
• 出版年：2014
• 出版时间：January 2014
• 年：2014
• 卷：33
• 期：1
• 页码：221-225
• 全文大小：208 KB
• 作者单位：Changpin Zhou (1) (2)
  Xudong He (3)
  Fagen Li (2)
  Qijie Weng (2)
  Xiaoli Yu (2)
  Yu Wang (2)
  Mei Li (2)
  Jisen Shi (3)
  Siming Gan (1) (2)
  
  1. State Key Laboratory of Tree Genetics and Breeding, Chinese Academy of Forestry, Xiangshan Road, Beijing, 100091, China
  2. Research Institute of Tropical Forestry, Chinese Academy of Forestry, Longdong, Guangzhou, 510520, China
  3. Key Laboratory of Forest Genetics and Biotechnology of Ministry of Education, Nanjing Forestry University, 159 Longpan Road, Nanjing, 210037, China
  
• ISSN：1572-9788

文摘

Simple sequence repeat (SSR) markers derived from expressed sequence tag (EST) resources provide great potential for comparative mapping, direct gene tagging of quantitative trait loci and functional diversity studies. Here we report on the development of 240 novel EST-SSRs for the important tree genus Eucalyptus L’Hérit. Of the 240 EST-SSR loci, 218 (90.8?%) were polymorphic among 12 individuals of E. grandis Hill ex Maiden, with the number of alleles per locus (N a), observed heterozygosity (H o), expected heterozygosity (H e) and polymorphic information content (PIC) averaging at 5.0, 0.403, 0.598 and 0.529, respectively. High rates of cross-species/subgenus amplification were observed. The EST-SSRs developed herein would be a valuable addition of functional markers for genetics and breeding applications in a wide range of eucalypt species. The primer sequences for the 240 EST-SSRs have been deposited in the Probe database of GenBank (IDs Pr016588534-73).