Immunophenotypic features and t(14;18) (q32;q21) translocation of Chinese follicular lymphomas helps to distinguish subgroups

详细信息    查看全文

• 作者：Fen Zhang (8)
  Li-Xu Yan (8)
  Su-Xia Lin (9)
  Zi-Yin Ye (10)
  Heng-Guo Zhuang (8)
  Jing-Ping Yun (9)
  Han-Liang Lin (10)
  Dong-Lan Luo (8)
  Fang-Ping Xu (8)
  Xin-Lan Luo (8)
  Jie Cheng (8)
  Ke-Ping Zhang (8)
  Yan-Hui Liu (8)
  
• 关键词：Follicular lymphoma ; CD10 ; BCL6 ; MUM1 ; BCL2 ; Immunophenotype ; t(14 ; 18) (q32 ; q21) translocation
• 刊名：Diagnostic Pathology
• 出版年：2013
• 出版时间：December 2013
• 年：2013
• 卷：8
• 期：1
• 全文大小：1,142 KB
• 参考文献：1. Groves FD, Linet MS, Travis LB, Devesa SS: Cancer surveillance series: non-Hodgkin’s lymphoma incidence by histologic subtype in the United States from 1978 through 1995. / J Natl Cancer Inst 2000,92(15):1240-251. CrossRef
  2. Bosga-Bouwer AG, van den Berg A, Haralambieva E, de Jong D, Boonstra R, Kluin P, van den Berg E, Poppema S: Molecular, cytogenetic, and immunophenotypic characterization of follicular lymphoma grade 3B; a separate entity or part of the spectrum of diffuse large B-cell lymphoma or follicular lymphoma? / Human pathology 2006,37(5):528-33. CrossRef
  3. Karube K, Guo Y, Suzumiya J, Sugita Y, Nomura Y, Yamamoto K, Shimizu K, Yoshida S, Komatani H, Takeshita M, Kikuchi M, Nakamura N, Takasu O, Arakawa F, Tagawa H, Seto M, Ohshima K: CD10-MUM1+ follicular lymphoma lacks BCL2 gene translocation and shows characteristic biologic and clinical features. / Blood 2007,109(7):3076-079.
  4. Ott G, Katzenberger T, Lohr A, Kindelberger S, Rudiger T, Wilhelm M, Kalla J, Rosenwald A, Muller JG, Ott MM, Muller-Hermelink HK: Cytomorphologic, immunohistochemical, and cytogenetic profiles of follicular lymphoma: 2 types of follicular lymphoma grade 3. / Blood 2002,99(10):3806-812. CrossRef
  5. Carbone A, Gloghini A, Aldinucci D, Gattei V, Dalla-Favera R, Gaidano G: Expression pattern of MUM1/IRF4 in the spectrum of pathology of Hodgkin’s disease. / Br J Haematol 2002,117(2):366-72. CrossRef
  6. Papakonstantinou G, Verbeke C, Hastka J, Bohrer M, Hehlmann R: bcl-2 expression in non-Hodgkin’s lymphomas is not associated with bcl-2 gene rearrangements. / Br J Haematol 2001,113(2):383-90. CrossRef
  7. Peh SC, Shaminie J, Tai YC, Tan J, Gan SS: The pattern and frequency of t(14;18) translocation and immunophenotype in Asian follicular lymphoma. / Histopathology 2004,45(5):501-10. CrossRef
  8. Horn H, Schmelter C, Leich E, Salaverria I, Katzenberger T, Ott MM, Kalla J, Romero M, Siebert R, Rosenwald A, Rosenwald A, Ott G: Follicular lymphoma grade 3B is a distinct neoplasm according to cytogenetic and immunohistochemical profiles. / Haematologica 2011,96(9):1327-334. CrossRef
  9. Einerson RR, Kurtin PJ, Dayharsh GA, Kimlinger TK, Remstein ED: FISH is superior to PCR in detecting t(14;18)(q32;q21)-IgH/bcl-2 in follicular lymphoma using paraffin-embedded tissue samples. / Am J Clin Pathol 2005,124(3):421-29. CrossRef
  10. Chang X, Zu Lan S, Qiu Liang W, Hong Yi G, Jian Chen F, Zhong Jun X, Zhong Zhen G: Clinical and pathological reassessment of 493 cases of non-Hodgkin’s lymphomas according to current WHO classification of lymphoid neoplasms. / Chin J Pathol 2005,34(1):22-7.
  11. Goteri G, Lucarini G, Zizzi A, Costagliola A, Giantomassi F, Stramazzotti D, Rubini C, Leoni P: Comparison of germinal center markers CD10, BCL6 and human germinal center-associated lymphoma (HGAL) in follicular lymphomas. / Diagn Pathol 2011, 6:97. CrossRef
  12. Xiao Qiu L, Di Gan L, Zi Fen G, Xiao Ge Z, Xiong Zeng Z, Group TCLS: Distribution pattern of lymphoma subtypes in China: A nationwide multicenter study of 10002 cases. / J Diagn Concepts Pract 2012,11(2):111-15.
  13. Swerdlow SH, Campo E, Harris NL, Jaffe ES, Pileri SA, Stein H, Thiele J, Vardiman JW: / WHO classification of tumors of haematopoietic and lymphoid tissues. 4th edition. France: IARC press Lyon; 2008:220-26.
  14. Yang QP, Zhang WY, Yu JB, Zhao S, Xu H, Wang WY, Bi CF, Zuo Z, Wang XQ, Huang J, Dai L, Liu WP: Subtype distribution of lymphomas in Southwest China: analysis of 6,382 cases using WHO classification in a single institution. / Diagn Pathol 2011, 6:77. CrossRef
  15. Hans CP, Weisenburger DD, Vose JM, Hock LM, Lynch JC, Aoun P, Greiner TC, Chan WC, Bociek RG, Bierman PJ, Armitage JO: A significant diffuse component predicts for inferior survival in grade 3 follicular lymphoma, but cytologic subtypes do not predict survival. / Blood 2003,101(6):2363-367. CrossRef
  16. Katzenberger T, Ott G, Klein T, Kalla J, Muller-Hermelink HK, Ott MM: Cytogenetic alterations affecting BCL6 are predominantly found in follicular lymphomas grade 3B with a diffuse large B-cell component. / Am J Pathol 2004,165(2):481-90. CrossRef
  17. Prevodnik VK, Lavrencak J, Horvat M, Novakovic BJ: The predictive significance of CD20 expression in B-cell lymphomas. / Diagn Pathol 2011, 6:33. CrossRef
  18. Hans CP, Weisenburger DD, Greiner TC, Gascoyne RD, Delabie J, Ott G, Muller-Hermelink HK, Campo E, Braziel RM, Jaffe ES, Pan Z, Farinha P, Smith LM, Falini B, Banham AH, Rosenwald A, Staudt LM, Connors JM, Armitage JO, Chan WC: Confirmation of the molecular classification of diffuse large B-cell lymphoma by immunohistochemistry using a tissue microarray. / Blood 2004,103(1):275-82. CrossRef
  19. Oh S, Koo DH, Suh C, Kim S, Park BH, Kang J, Huh J: Prognostic value of immunohistochemical biomarkers at different cut-off values in patients with diffuse large B-cell lymphoma treated with CHOP chemotherapy. / J Korean med sci 2011,26(12):1556-562. CrossRef
  20. Bende RJ, Smit LA, van Noesel CJ: Molecular pathways in follicular lymphoma. / Leukemia 2007,21(1):18-9. CrossRef
  21. Ngan BY, Chen-Levy Z, Weiss LM, Warnke RA, Cleary ML: Expression in non-Hodgkin’s lymphoma of the bcl-2 protein associated with the t(14;18) chromosomal translocation. / N Engl J Med 1988,318(25):1638-644. CrossRef
  22. Lai R, Arber DA, Chang KL, Wilson CS, Weiss LM: Frequency of bcl-2 expression in non-Hodgkin’s lymphoma: a study of 778 cases with comparison of marginal zone lymphoma and monocytoid B-cell hyperplasia. / Mod Pathol 1998,11(9):864-69.
  23. Kishimoto K, Kitamura T, Fujita K, Tate G, Mitsuya T: Cytologic differential diagnosis of follicular lymphoma grades 1 and 2 from reactive follicular hyperplasia: cytologic features of fine-needle aspiration smears with Pap stain and fluorescence in situ hybridization analysis to detect t(14;18)(q32;q21) chromosomal translocation. / Diagn Cytopathol 2006,34(1):11-7. CrossRef
  24. Sekiguchi N, Kobayashi Y, Yokota Y, Kusumoto S, Tanimoto K, Watanabe T, Matsuno Y, Tobinai K: Follicular lymphoma subgrouping by fluorescence in situ hybridization analysis. / Cancer sci 2005,96(2):77-2. CrossRef
  25. Gollub W, Stassek B, Huckhagel T, Bernd HW, Krokowski M, Merz H, Feller AC, Thorns C: BCL6-translocations affect the phenotype of follicular lymphomas only in the absence of t(14;18)IgH/BCL2. / Anticancer Res 2009,29(11):4649-655.
  26. Liu YH, Xu FP, Zhuang HG, Lai KC, Xie D, Luo DL, Li L, Luo XL, Xu J, Zhang MH, Zhang F, Li HM: Clinicopathologic significance of immunophenotypic profiles related to germinal center and activation B-cell differentiation in diffuse large B-cell lymphoma from Chinese patients. / Hum Pathol 2008,39(6):875-84. CrossRef
  27. Vega F, Medeiros LJ: Chromosomal translocations involved in non-Hodgkin lymphomas. / Arch Pathol Lab Med 2003,127(9):1148-160.
  28. Schraders M, de Jong D, Kluin P, Groenen P, van Krieken H: Lack of Bcl-2 expression in follicular lymphoma may be caused by mutations in the BCL2 gene or by absence of the t(14;18) translocation. / J Pathol 2005,205(3):329-35. CrossRef
  29. Jost PJ, Ruland J: Aberrant NF-kappaB signaling in lymphoma: mechanisms, consequences, and therapeutic implications. / Blood 2007,109(7):2700-707.
  30. Bosga-Bouwer AG, van Imhoff GW, Boonstra R, van der Veen A, Haralambieva E, van den Berg A, de Jong B, Krause V, Palmer MC, Coupland R, Kluin PM, van den Berg E, Poppema S: Follicular lymphoma grade 3B includes 3 cytogenetically defined subgroups with primary t(14;18), 3q27, or other translocations: t(14;18) and 3q27 are mutually exclusive. / Blood 2003,101(3):1149-154. CrossRef
  
• 作者单位：Fen Zhang (8)
  Li-Xu Yan (8)
  Su-Xia Lin (9)
  Zi-Yin Ye (10)
  Heng-Guo Zhuang (8)
  Jing-Ping Yun (9)
  Han-Liang Lin (10)
  Dong-Lan Luo (8)
  Fang-Ping Xu (8)
  Xin-Lan Luo (8)
  Jie Cheng (8)
  Ke-Ping Zhang (8)
  Yan-Hui Liu (8)
  
  8. Department of Pathology, Guangdong General Hospital, Guangdong Academy of Medical Science, Guangzhou, 510080, China
  9. Department of Pathology, Sun Yat-Sen University Cancer Center, Guangzhou, 510060, China
  10. Department of Pathology, the First Affiliated Hospital of Sun Yat-Sen University, Guangzhou, 510060, China
  
• ISSN：1746-1596

文摘

Background The revised 2008 World Health Organization classification maintains a histological grading system (grades 1-) for follicular lymphoma (FL). The value of grading FL has been debated. This study will yield deeper insights into the morphologic, immunophenotypic characterization and t(14;18) translocation in FL and explore their significance of diagnosis of Chinese FL subgroups. Methods We retrospectively reviewed the FL diagnoses according to the 2008 WHO classification in all diagnostic specimens from a multicentric cohort of 122 Chinese patients. Upon review, 115 cases proved to be truly FL. CD10, BCL6, MUM1, BCL2 and t(14;18) (q32;q21) translocation were detected by Envision immunostaining technique and fluorescence in situ hybridization. Results FL1 has larger proportion of follicular pattern (93.0%) than that of FL2 (73.7%, P = 0.036), FL3B (63.6%, P = 0.003) and FL3A (77.4%, P = 0.053), although the last P value was more than 0.05 (Pearson’s chi-squared test). Areas of DLBCL were present in 25.8% (8/31) of FL3A and more frequent in FL3B (59.1%, 13/22; P = 0.015). The positivity of CD10 and BCL2 in FL1-2 were significantly higher than those in FL3 (P < 0.001, P = 0.043, respectively). The positivity of MUM1 in FL1-2 was significantly lower than that in FL3 (10.2% vs. 51.0%; P < 0.001). Furthermore the positivity of MUM1 in FL3A was significantly lower than that in FL3B (37.9% vs. 68.2%; P = 0.032). The positivity of t(14;18) was higher in FL1-2 than in FL3 (73.5% vs. 35.6%, P < 0.001), and was higher in FL3A than in FL3B (51.9% vs. 11.1%, P = 0.005). t(14;18) was significantly correlated with CD10+ (R = 0.453, P < 0.001) and MUM1+ (R = -0.482, P < 0.001). Conclusions FL1 and FL2 were immunophenotypically and genomically similar, while FL3A and FL3B were partly immunophenotypically similar but morphologically, genomically distinct. FL3A was genomically closer to FL1-2, whereas FL3A was genomically closer DLBCL. Thus we hypothesize that FL may in fact be a heterogeneous indolent lymphoma encompassing entities with distinct molecular pathogenesis and genetic characteristics. Immunohistochemical and genetic characterization helps to distinguish subgroups of FLs. Virtual slides The virtual slide(s) for this article can be found here: http://www.diagnosticpathology.diagnomx.eu/vs/1334018129864616.