Gene analysis of an antiviral protein SP-2 from Chinese wild silkworm, Bombyx mandarina Moore and its bioactivity assay
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- 作者：HuiPeng Yao (1)
FangQing He (1)
AiQin Guo (1)
CuiPing Cao (1)
XingMeng Lu (1)
XiaoFeng Wu (1) - 关键词：Bombyx mandarina Moore ; antiviral protein ; bioactivity assay
- 刊名：Science China Life Sciences
- 出版年：2008
- 出版时间：October 2008
- 年：2008
- 卷：51
- 期：10
- 页码：879-884
- 全文大小：888KB
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FangQing He (1)
AiQin Guo (1)
CuiPing Cao (1)
XingMeng Lu (1)
XiaoFeng Wu (1)
1. College of Animal Science, Zhejiang University, Hangzhou, 310029, China
文摘
The cDNA encoding an antiviral protein SP-2 against BmNPV was cloned from the midgut of Chinese wild silkworm, Bombyx mandarina Moore (GenBank access AY945210) based on the available information of the domesticated silkworm. Its cDNA was 855 bp encoding 284 amino acids with predicted molecular weight of 29.6 kDa. Its full length in genomics was 1376 bp, including 5 exons and 4 introns. The expression analysis indicated that it was only expressed in midgut, and its expression level was higher during feeding stage of larval instars while very lower during the moltism and mature stages. The deduced amino acid sequence of this protein showed eight-amino-acid variation compared with the counterpart of domesticated silkworm. Its antiviral activity was assayed through in vitro test. The results indicated that it showed strong bioactivity against BmNPV, and its activity was 1.6 fold higher that the counterpart of domesticated silkworm.