A higher in value biopolymer product of polyhydroxyalkanoates (PHAs) synthesized by Alcaligenes latus in batch/repeated batch fermentation processes of sugar cane juice

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• 作者：Pakjirat Singhaboot ; Pakawadee Kaewkannetra
• 关键词：Polyhydroxyalkanoates ; Alcaligenes latus ; Sugar cane juice ; Fermentation process
• 刊名：Annals of Microbiology
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：65
• 期：4
• 页码：2081-2089
• 全文大小：657 KB
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• 作者单位：Pakjirat Singhaboot (1)
  Pakawadee Kaewkannetra (2)
  
  1. Graduate School of Khon Kaen University, Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand
  2. Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen, 40002, Thailand
  
• 刊物主题：Microbiology; Microbial Genetics and Genomics; Microbial Ecology; Fungus Genetics; Medical Microbiology; Applied Microbiology;
• 出版者：Springer Berlin Heidelberg
• ISSN：1869-2044

文摘

Biodegradable polymers of polyhydroxyalkanoates (PHAs) are produced and accumulated in various microorganisms, especially in bacteria. In this work, a cheap agricultural sugar cane juice (SCJ) was used as a carbon source by Alcaligenes latus during its growth coupled with the production of PHAs via batch/ repeated batch fermentation processes. In batch fermentation, the maximum PHAs and PHAs content of 2.20?±-.14 g/L and 53.65 % were obtained within 24 h while in the case of four cycles of repeated batch fermentation, the PHAs productivity was reached at about 0.21 g/L·h that was 2.33 fold when compared to the first batch fermentation (0.09 g/L·h). Interestingly, the duration in each cycle or in batch operation during repeated batch fermentation was shorter than that found in the batch fermentation and PHAs productivity was also improved. After PHAs recovery, the physicochemical properties of nuclear magnetic resonance (NMR) and a fourier transform infrared (FTIR) spectrogram were characterized and revealed to be in full agreement with the standard polymer of polyhydroxybutyrate (PHB). Keywords Polyhydroxyalkanoates Alcaligenes latus Sugar cane juice Fermentation process