Candida bracarensis: Evaluation of Virulence Factors and its Tolerance to Amphotericin B and Fluconazole

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• 作者：André Moreira ; Sónia Silva ; Cláudia Botelho ; Paula Sampaio ; Célia Pais…
• 关键词：Candida bracarensis ; Virulence factors ; Antifungal resistance ; Amphotericin B ; Fluconazole
• 刊名：Mycopathologia
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：180
• 期：5-6
• 页码：305-315
• 全文大小：1,072 KB
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• 作者单位：André Moreira (1)
  Sónia Silva (1)
  Cláudia Botelho (1)
  Paula Sampaio (2)
  Célia Pais (2)
  Mariana Henriques (1)
  
  1. CEB, Centre of Biological Engineering, LIBRO -Laboratório de investiga??o em Biofilmes Rosário Oliveira, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal
  2. CBMA, Centre of Molecular and Environmental Biology, Department of Biology, University of Minho, Campus de Gualtar, 4710-057, Braga, Portugal
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Medical Microbiology
  Plant Sciences
  Microbial Ecology
  
• 出版者：Springer Netherlands
• ISSN：1573-0832

文摘

Candida bracarensis is an uncommon Candida species found during an epidemiological study of candidiasis performed in Braga, Portugal. Initially, it was identified as C. glabrata, but recently detailed analyses pointed out their differences. So, little information is still available about C. bracarensis virulence factors and antifungal susceptibilities. Therefore, the main goal of this work is to evaluate the ability of C. bracarensis to form biofilms, to produce hydrolytic enzymes (proteases, phospholipases and hemolysins), as well as its susceptibility to amphotericin B and fluconazole. It was shown, for the first time, that all C. bracarensis strains were able to form biofilms and display proteinase and hemolytic activities. Moreover, although planktonic cells presented antifungal susceptibility, amphotericin B and fluconazole were unable to inhibit biofilm formation and eradicate pre-formed biofilms. Due to the propensity of C. bracarensis to display antifungal resistance and virulence attributes, the control of these emerging pathogens is recommended. Keywords Candida bracarensis Virulence factors Antifungal resistance Amphotericin B Fluconazole