The Role of UPC2 Gene in Azole-Resistant Candida tropicalis

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• 作者：Cen Jiang ; Qi Ni ; Danfeng Dong ; Lihua Zhang ; Zhen Li ; Yuan Tian…
• 关键词：UPC2 ; Azole ; Antifungal resistance ; Candida tropicalis
• 刊名：Mycopathologia
• 出版年：2016
• 出版时间：December 2016
• 年：2016
• 卷：181
• 期：11-12
• 页码：833-838
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Microbiology; Medical Microbiology; Eukaryotic Microbiology; Microbial Ecology; Plant Sciences;
• 出版者：Springer Netherlands
• ISSN：1573-0832
• 卷排序：181

文摘

Azole resistance of Candida tropicalis has, in recent years, become a serious issue in hospitals; however, there is limited knowledge of the mechanisms underlying this resistance. We have previously demonstrated that ERG11 plays a vital role in azole resistance in C. tropicalis. Here, we describe the expression and sequence variation of UPC2, which encodes a transcription factor of ERG11. Quantitative real-time RT-PCR showed that 31 azole-resistant C. tropicalis strains significantly overexpressed UPC2. Those isolates resistant to all three azole antifungals upregulated UPC2 expression to a greater degree than those resistant to only fluconazole or itraconazole. The UPC2 promoter contains mutations −118T-G and −155G-A in azole-resistant strains of C. tropicalis. Meanwhile, the mutation G392E was also detected twice in UPC2 gene in azole-resistant C. tropicalis and was demonstrated to mediate azole antifungal susceptibility by using Saccharomyces cerevisiae as an expression host, particularly for fluconazole and itraconazole.