Increased synthesis of folate transporters regulates folate transport in conditions of ethanol exposure and folate deficiency
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- 作者：Shilpa Thakur ; Deepti More ; Beenish Rahat…
- 关键词：Folate transport ; Folate transporters ; HEK293 cells ; Ethanol exposure ; Folate deficiency ; Regulatory mechanisms
- 刊名：Molecular and Cellular Biochemistry
- 出版年：2016
- 出版时间：January 2016
- 年：2016
- 卷：411
- 期：1-2
- 页码：151-160
- 全文大小：2,060 KB
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Deepti More (1)
Beenish Rahat (1)
Krishan Lal Khanduja (2)
Jyotdeep Kaur (1)
1. Department of Biochemistry, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India
2. Department of Biophysics, Postgraduate Institute of Medical Education and Research, Chandigarh, 160012, India - 刊物类别：Biomedical and Life Sciences
- 刊物主题：Life Sciences
Biochemistry
Medical Biochemistry
Oncology
Cardiology - 出版者：Springer Netherlands
- ISSN：1573-4919
文摘
Excessive alcohol consumption and dietary folate inadequacy are the main contributors leading to folate deficiency (FD). The present study was planned to study regulation of folate transport in conditions of FD and ethanol exposure in human embryonic kidney cell line. Also, the reversible nature of effects mediated by ethanol exposure and FD was determined by folate repletion and ethanol removal. For ethanol treatment, HEK293 cells were grown in medium containing 100 mM ethanol, and after treatment, one group of cells was shifted on medium that was free from ethanol. For FD treatment, cells were grown in folate-deficient medium followed by shifting of one group of cells on folate containing medium. FD as well as ethanol exposure resulted in an increase in folate uptake which was due to an increase in expression of folate transporters, i.e., reduced folate carrier, proton-coupled folate transporter, and folate receptor, both at the mRNA and protein level. The effects mediated by ethanol exposure and FD were reversible on removal of treatment. Promoter region methylation of folate transporters remained unaffected after FD and ethanol exposure. As far as transcription rate of folate transporters is concerned, an increase in rate of synthesis was observed in both ethanol exposure and FD conditions. Additionally, mRNA life of folate transporters was observed to be reduced by FD. An increased expression of folate transporters under ethanol exposure and FD conditions can be attributed to enhanced rate of synthesis of folate transporters. Keywords Folate transport Folate transporters HEK293 cells Ethanol exposure Folate deficiency Regulatory mechanisms