Autophagy-related and autophagy-regulatory genes are induced in human muscle after ultraendurance exercise

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• 作者：Cécile Jamart (1)
  Nicolas Benoit (1)
  Jean-Marc Raymackers (1)
  Hyo Jeong Kim (2)
  Chang Keun Kim (2)
  Marc Francaux (1) marc.francaux@uclouvain.be
• 关键词：Endurance exercise – Autophagy – ATG – BNIP3 – Gabarapl1 – LC3b
• 刊名：European Journal of Applied Physiology
• 出版年：2012
• 出版时间：August 2012
• 年：2012
• 卷：112
• 期：8
• 页码：3173-3177
• 全文大小：248.3 KB
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• 作者单位：1. Institute of Neuroscience, Research Group in Muscle and Exercise Physiology, Université catholique de Louvain, Place Pierre de Coubertin 1, 1348 Louvain-la-Neuve, Belgium2. Department of Aging and Human Physiology, Korea National Sport University, 88-15 Oryun-dong, Songpa-gu, Seoul, South Korea
• ISSN：1439-6327

文摘

The purpose of this study was to evaluate whether ultra endurance exercise changes the mRNA levels of the autophagy-related and autophagy-regulatory genes. Eight men (44 ± 1 years, range: 38–50 years) took part in a 200-km running race. The average running time was 28 h 03 min ± 2 h 01 min (range: 22 h 15 min–35 h 04 min). A muscle sample was taken from the vastus lateralis 2 weeks prior to the race and 3 h after arrival. Gene expression was assessed by RT-qPCR. Transcript levels of autophagy-related genes were increased by 49% for ATG4b (P = 0.025), 57% for ATG12 (P = 0.013), 286% for Gabarapl1 (P = 0.008) and 103% for LC3b (P = 0.011). The lysosomal enzyme cathepsin L mRNA was upregulated by 123% (P = 0.003). Similarly, transcript levels of the autophagy-regulatory genes BNIP3 and BNIP3l were both increased by 113% (P = 0.031 and P = 0.007, respectively). Since upregulation of these genes has been related with an increased autophagic flux in various models, our results strongly suggest that autophagy is activated in response to ultra endurance exercise.