Jasmonic acid-induced hydrogen peroxide activates MEK1/2 in upregulating the redox states of ascorbate and glutathione in wheat leaves

详细信息    查看全文

• 作者：Huiping Dai ; Genliang Jia ; Changjuan Shan
• 关键词：Hydrogen peroxide ; MEK1/2 ; Jasmonic acid ; Ascorbate ; Glutathione
• 刊名：Acta Physiologiae Plantarum
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：37
• 期：10
• 全文大小：517 KB
• 参考文献：Ai L, Li ZH, Xie ZX, Tian XL, Eneji AE, Duan LS (2008) Coronatine alleviates polyethylene glycol-induced water stress in two rice (Oryza sativa L.) cultivars. J Agron Crop Sci 194:360-68CrossRef
  Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72:248-54CrossRef PubMed
  Favata MF, Horiuchi KY, Manos EJ, Daulerio AJ, Stradley DA, Feeser WS, Van Dyk DE, Pitts WJ, Earl RA, Hobbs F, Copeland RA, Magolda RL, Scherle PA, Trzaskos JM (1998) Identification of a novel inhibitor of mitogen-activated protein kinase kinase. J Biol Chem 273:18623-8632CrossRef PubMed
  Grace SC, Logan BA (1996) Acclimation of foliar antioxidant systems to growth irradiance in three broad-leaved evergreen species. Plant Physiol 112:1631-640PubMed Central PubMed
  Griffith OW (1980) Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridine. Anal Biochem 106:207-12CrossRef PubMed
  Hodges DM, Andrews CJ, Johnson DA, Hamilton RI (1996) Antioxidant compound responses to chilling stress in differentially sensitive inbred maize lines. Plant Physiol 98:685-92CrossRef
  Hou Z, Liu J, Hou L, Li X, Liu X (2011) H2S may function downstream of H2O2 in jasmonic acid-induced stomatal closure in Vicia faba. Chin Bull Bot 46:396-06CrossRef
  Hu X, Jiang M, Zhang A, Lu J (2005) Abscisic acid-induced apoplastic H2O2 accumulation up-regulates the activities of chloroplastic and cytosolic antioxidant enzymes in maize leaves. Planta 223:57-8CrossRef PubMed
  Jiang M, Zhang J (2002) Water stress-induced abscisic acid accumulation triggers the increased generation of reactive oxygen species and up-regulates the activities of antioxidant enzymes in maize leaves. J Exp Bot 53:2401-410CrossRef PubMed
  Liu ZJ, Guo YK, Lin SH, Bai JG (2009) Effects of exogenous hydrogen peroxide on ultra structure of chloroplasts and activities of antioxidant enzymes in greenhouse-ecotype cucumber under drought stress. Acta Horti Sin 36:1140-146
  Liu Y, Zhang D, Wang L, Li D (2013) Genome-wide analysis of mitogen-activated protein kinase gene family in maize. Plant Mol Biol Rep 31:1446-460CrossRef
  Mahabub Alam M, Nahar K, Hasanuzzaman M, Fujita M (2014) Exogenous jasmonic acid modulates the physiology, antioxidant defense and glyoxalase systems in imparting drought stress tolerance in different?Brassica species. Plant Biotechnol Rep 8:279-93CrossRef
  Maksymiec W (2011) Effects of jasmonate and some other signalling factors on bean and onion growth during the initial phase of cadmium action. Biol Plant 55:112-18CrossRef
  Noctor G, Foyer CH (1998) Ascorbate and glutathione: keeping active oxygen under control. Annu Rev Plant Physiol 49:249-79CrossRef
  Pukacka S, Ratajczak E (2010) Ascorbate and glutathione metabolism during development and desiccation of beech (Fagus sylvatica?L.) seeds. Plant Growth Regul 62:77-3CrossRef
  Rüegsegger A, Brunold C (1992) Effect of cadmium on γ-glutamylcysteine synthesis in maize seedlings. Plant Physiol 99:428-33PubMed Central CrossRef PubMed
  Shan C, Liang Z (2010) Jasmonic acid regulates ascorbate and glutathione metabolism in Agropyron cristatum leaves under water stress. Plant Sci 178:130-39CrossRef
  Shan C, Zhao X (2014) Effects of lanthanum on the ascorbate and glutathione metabolism of?Vigna radiate?seedlings under salt stress. Biol Plant 58:595-99CrossRef
  Shan C, Liang Z, Sun Y, Hao W, Han R (2011) The protein kinase MEK1/2 participates in the regulation of ascorbate and glutathione content by jasmonic acid in Agropyron cristatum leaves. J Plant Physiol 168:514-18CrossRef PubMed
  Singh I, Shah K (2014a) Exogenous application of methyl jasmonate lowers the effect of cadmium-induced oxidative injury in rice seedlings. Phytochemistry 108:57-6CrossRef PubMed
  Singh I, Shah K (2014b) Evidences for structural basis of altered ascorbate peroxidase activity in cadmium-stressed rice plants exposed to jasmonate. Biometals 27:247-63CrossRef PubMed
  Suhita D, Raghavendra AS, Kwak JM, Vavasseur A (2004) Cytoplasmic alkalization precedes reactive oxygen species production during methyl jasmonate- and abscisic acid-induced stomatal closure. Plant Physiol 134:1536-545PubMed Central CrossRef PubMed
  Wen Y, Li X, Guo C, Ma C, Duan W, Lu W, Xiao K (2015) Characterization and expression analysis of mitogen-activated protein kinase cascade genes in wheat subjected to phosphorus and nitrogen deprivation, high salinity, and drought. J Plant Biochem Biot 24:184-96CrossRef
  Zhang S, Klessig DF (1997) Salicylic acid activates a 48-kD MAP kinase in tobacco. Plant Cell 9:809-24PubMed Central CrossRef PubMed
  Zhang A, Jiang M, Zhang J, Tan M, Hu X (2006) Mitogen-activated protein kinase is Involved in abscisic acid-induced antioxidant defense and acts downstream of reactive oxygen species production in leaves of maize plants
• 作者单位：Huiping Dai (1)
  Genliang Jia (2)
  Changjuan Shan (3)
  
  1. Shaanxi Province Key Laboratory of Bio-resources, Shaanxi University of Technology, Hanzhong, 723001, China
  2. College of Science, Northwest A&F University, Yangling, 712100, China
  3. Henan Institute of Science and Technology, Xinxiang, 453003, China
  
• 刊物主题：Plant Physiology; Plant Genetics & Genomics; Plant Biochemistry; Plant Pathology; Plant Anatomy/Development; Agriculture;
• 出版者：Springer Berlin Heidelberg
• ISSN：1861-1664

文摘

We studied the relationship between hydrogen peroxide (H₂O₂) and MEK1/2 in jasmonic acid (JA) signaling in regulating the redox states of ascorbate (AsA) and glutathione (GSH). JA increased H₂O₂ production, MEK1/2 phosphorylation, the transcription levels and activities of AsA and GSH metabolic enzymes (APX, GR, DHAR, MDHAR, GalLDH and γ-ECS), AsA and GSH contents, and the redox states of AsA and GSH (ratios of AsA/DHA and GSH/GSSG). Above increases were inhibited by applications of H₂O₂ synthesis inhibitor DPI and scavenger DMTU. However, applications of MEK1/2 inhibitors PD98059 and U0126 had no significant effect on JA-induced H₂O₂ production. Treatments with exogenous H₂O₂ also increased MEK1/2 phosphorylation, the transcription levels and activities of AsA and GSH metabolic enzymes, AsA and GSH contents, and the redox states of AsA and GSH. Above increases except the transcription level and activity of γ-ECS were all suppressed by pretreatments with PD98059 and U0126. Our results suggested that JA-induced H₂O₂ could trigger MEK1/2 phosphorylation and activation leading to the upregulation of AsA and GSH metabolic enzymes. Keywords Hydrogen peroxide MEK1/2 Jasmonic acid Ascorbate Glutathione