Decreased microbial diversity and Lactobacillus group in the intestine of geriatric giant pandas (Ailuropoda melanoleuca)

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• 作者：Zhirong Peng ; Dong Zeng ; Qiang Wang…
• 关键词：Captive giant panda ; Diet ; Intestine microflora ; PCR ; DGGE ; Real ; time PCR
• 刊名：World Journal of Microbiology & Biotechnology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：32
• 期：5
• 全文大小：568 KB
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• 作者单位：Zhirong Peng (1)
  Dong Zeng (2)
  Qiang Wang (3)
  Lili Niu (3)
  Xueqin Ni (1)
  Fuqin Zou (1)
  Mingyue Yang (2)
  Hao Sun (2)
  Yi Zhou (1)
  Qian Liu (2)
  Zhongqiong Yin (2)
  Kangcheng Pan (1)
  Bo Jing (1)
  
  1. Animal Microecology Institute, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, 611130, China
  2. Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, 611130, China
  3. Chengdu Wildlife Institute, Chengdu Zoo, Chengdu, 610081, China
  
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Applied Microbiology
  Biotechnology
  Biochemistry
  Environmental Biotechnology
  Microbiology
  
• 出版者：Springer Netherlands
• ISSN：1573-0972

文摘

It has been established beyond doubt that giant panda genome lacks lignin-degrading related enzyme, gastrointestinal microbes may play a vital role in digestion of highly fibrous bamboo diet. However, there is not much information available about the intestinal bacteria composition in captive giant pandas with different ages. In this study, we compared the intestinal bacterial community of 12 captive giant pandas from three different age groups (subadults, adults, and geriatrics) through PCR-denaturing gradient gel electrophoresis (DGGE) and real-time PCR analysis. Results indicated that microbial diversity in the intestine of adults was significantly higher than that of the geriatrics (p < 0.05), but not significant compared to the subadults (p > 0.05). The predominant bands in DGGE patterns shared by the twelve pandas were related to Firmicutes and Proteobacteria. Additionally, in comparison to healthy individuals, antibiotic-treated animals showed partial microbial dysbiosis. Real-time PCR analyses confirmed a significantly higher abundance of the Lactobacillus in the fecal microbiota of adults (p < 0.05), while other bacterial groups and species detected did not significantly differ among the three age groups (p > 0.05). This study revealed that captive giant pandas with different ages showed different intestinal bacteria composition.