Chryseobacterium chengduensis sp. nov. isolated from the air of captive giant panda enclosures in Chengdu, China

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• 作者：Cai-fang Wen ; Li-xin Xi ; Shan Zhao…
• 刊名：Journal of Zhejiang University SCIENCE B
• 出版年：2016
• 出版时间：August 2016
• 年：2016
• 卷：17
• 期：8
• 页码：610-618
• 全文大小：712 KB
• 刊物主题：Biomedicine general;
• 出版者：Springer Berlin Heidelberg
• ISSN：1862-1783
• 卷排序：17

文摘

A Gram-negative, aerobic, non-motile, rod-shaped bacterial strain, designated 25-1T, was isolated from the air inside giant panda enclosures at the Chengdu Research Base of Giant Panda Breeding, China. Strain 25-1T grew optimally at pH 7.0–8.0, at 28–30 °C and in the presence of NaCl concentrations from 0.0% to 0.5 %. 16S rRNA gene sequence analysis indicated that strain 25-1T belongs to the genus Chryseobacterium within the family Flavobacteriaceae and is related most closely to C. carnis G81T (96.4% similarity), C. lathyri RBA2-6T (95.8% similarity), and C. zeae JM1085T (95.8% similarity). Its genomic DNA G+C molar composition was 36.2%. The major cellular fatty acids were iso-C_15:0 (44.0%), iso-C_17:0 3OH (19.8%) and C_16:1 ω7c/_16:1 ω6c (12.7%). The only isoprenoid quinone was menaquinone 6 (MK-6). The major polar lipids were phosphatidylethanolamine, two unidentified amino lipids and two unidentified lipids. The DNA–DNA relatedness between strain 25-1T and C. lathyri RBA2-6T was 38%. Phenotypic, genotypic, and phylogenetic characteristics indicated that strain 25-1T is a novel member of the genus Chryseobacterium, for which the name C. chengduensis sp. nov. is proposed. The type strain is 25-1T (CCTCC AB2015133T=DSM 100396T).Key wordsChryseobacterium chengduensisGiant panda16S rRNA sequencingPhylogenetic analysisStrain 25-1T