Mortality and ostreid herpesvirus 1 infection in the pacific oyster Crassostrea gigas in the Gulf of La Spezia, Italy

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• 作者：Laura Serracca ; Irene Rossini ; Roberta Battistini…
• 关键词：Vibrio ; Ostreid herpesvirus 1 ; Crassostrea gigas
• 刊名：Aquaculture International
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：24
• 期：1
• 页码：199-209
• 全文大小：421 KB
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• 作者单位：Laura Serracca (1)
  Irene Rossini (1)
  Roberta Battistini (1)
  Carlo Ercolini (1)
  
  1. Marine Microbiology Laboratory of the Experimental Zooprophylactic Institute of Piemonte Liguria and Valle d’Aosta, La Spezia, Italy
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Hydrobiology
  
• 出版者：Springer Netherlands
• ISSN：1573-143X

文摘

Between May 2011 and February 2014, 205 samples of Crassostrea gigas, farmed on the northwest coast of Italy, were collected and analyzed for OsHV-1, Vibrio parahaemolyticus, V. cholerae, V. alginolyticus, V. aestuarianus, bacteria belonging to V. splendidus clade, E. coli, Salmonella spp., environmental parameters and algae. Statistical analysis was carried out to correlate all the data of the environmental parameters, algae and Vibrio with OsHV-1 in the presence and absence of die-off. Of the samples tested, 46.8 % (96/205) were positive for the detection of OsHV-1 DNA, 0.97 % (2/205) of the oysters were positive for V. harveyi DNA detection and 8.8 % (18/205) were positive for V. aestuarianus. Statistical analysis showed a positive correlation between the presence of V. aestuarianus, OsHV-1 and a die-off occurred in November 2013. Sequencing analysis of the PCR products revealed that the genotype of OsHV-1 is highly related to the variant µVar. Keywords Vibrio Ostreid herpesvirus 1 Crassostrea gigas