Multiplex PCR assay using SCAR primers to detect Eimeria spp. in chicken

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• 作者：P. Venkateswara Rao (1) (3)
  M. Raman (1)
  G. DhinakarRaj (2)
  S. Abdul Basith (1)
  S. Gomathinayagam (1)
  
• 关键词：Mixed spectrum ; Eimeria ; Morphometry ; Faecal harvest ; Multiplex PCR ; SCAR
• 刊名：Journal of Parasitic Diseases
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：37
• 期：1
• 页码：110-113
• 全文大小：244KB
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• 作者单位：P. Venkateswara Rao (1) (3)
  M. Raman (1)
  G. DhinakarRaj (2)
  S. Abdul Basith (1)
  S. Gomathinayagam (1)
  
  1. Department of Veterinary Parasitology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007, India
  3. Department of Veterinary Parasitology, College of Veterinary Science, Sri Venkateswara Veterinary University, Korutla, 505 326, Andhra Pradesh, India
  2. Department of Animal Biotechnology, Madras Veterinary College, Tamil Nadu Veterinary & Animal Sciences University, Vepery, Chennai, 600 007, India
  

文摘

About 11 faecal samples from various regions of Tamil Nadu and Andhra Pradesh containing mixed spectrum of Eimeria species detected by morphometry viz. 15.4?×?11.2, 28.5?×?20.3, 31.1?×?18.5, 13.2?×?12.4, 20.8?×?17.5 and 22?×?18?μm for E. acervulina, E. brunetti, E. maxima, E. mitis, E. necatrix, E. praecox and E. tenella respectively were taken for the study. The oocysts were concentrated and purified using faecal harvest protocol. The genomic DNA is extracted as per kit protocol. The amplicons of sizes 539?bp (E. tenella) and 460?bp (E. mitis) obtained could be visualised in a single lane for the multiplex PCR assay using sequence characterized amplified region primers.