A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes

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• 作者：Anyarat Thanapipatsiri ; Jan Claesen ; Juan-Pablo Gomez-Escribano…
• 关键词：Streptomyces ; Expression host ; Type III polyketide synthases
• 刊名：Microbial Cell Factories
• 出版年：2015
• 出版时间：December 2015
• 年：2015
• 卷：14
• 期：1
• 全文大小：2,416 KB
• 刊物类别：Chemistry and Materials Science
• 刊物主题：Biotechnology
  Applied Microbiology
  Environmental Engineering/Biotechnology
  
• 出版者：BioMed Central
• ISSN：1475-2859

文摘

Background Recent advances in genome sequencing, combined with bioinformatic analysis, has led to the identification of numerous novel natural product gene clusters, particularly in actinomycetes of terrestrial and marine origin. Many of these gene clusters encode uncharacterised Type III polyketide synthases. To facilitate the study of these genes and their potentially novel products, we set out to construct an actinomycete expression host specifically designed for the heterologous expression of Type III PKS genes and their gene clusters.