Studies on Interaction of Norfloxacin, Cu²⁺, and DNA by Spectral Methods

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• 作者：Gongwu Song ; Qiujun Yan and Yu He
• 关键词：Norfloxacin ; DNA ; Cu2+ ; fluorescence ; static ; quenching style
• 刊名：Journal of Fluorescence
• 出版年：2005
• 出版时间：September 2005
• 年：2005
• 卷：15
• 期：5
• 页码：673-678
• 全文大小：110 KB

文摘

The interactions of norfloxacin (NFA), DNA, and Cu2+ are studied by fluorescence and UV-spectra method. According to the experimental results, it can be concluded that NFA can form a steady binary complex with Cu2+. There is a linear relationship between the Fluorescence intensity of the norfloxacin–Cu2+–DNA system and the concentration of DNA. And when the concentration of the NFA is 1.95¡Á10-5 mol L-1, they possess a good linearity in the concentration of DNA ranged from 4.7¡Á10-6 to 2.8¡Á10-5 mol L-1.It is a good method due to the high sensitivity and selectivity.