At-line measurement of lactose in dairy-processing plants

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• 作者：Nick Glithero (1)
  Claire Clark (1)
  Lo Gorton (2)
  Wolfgang Schuhmann (3)
  Neil Pasco (1)
  
• 关键词：At ; line process analysis ; Lactose measurement ; Screen ; printed electrodes ; CDH ; based biosensors ; Flow injection analysis
• 刊名：Analytical and Bioanalytical Chemistry
• 出版年：2013
• 出版时间：April 2013
• 年：2013
• 卷：405
• 期：11
• 页码：3791-3799
• 全文大小：422KB
• 参考文献：1. Griffin M (2012) Food outlook global market analysis. Food and Agriculture Organisation UN 51-4
  2. Pedersen LD (1991) Assessment of sensors used in the food industry. Food Control 2:87-8 CrossRef
  3. Luong JHT, Male KB, Glennon JD (2008) Technology push versus market pull. Biosens Advances 26:492-00
  4. Velasco-Garcia MN, Mottram T (2003) Biosensor technology addressing agricultural problems. Biosyst Eng 84:1-2 CrossRef
  5. Marrakchi M, Dzyadevych SV, Lagarde F, Martelet C, Jaffrezic-Renault N (2008) Conductometric biosensor based on glucose oxidase and beta-galactosidase for specific lactose determination in milk. Mater Sci Eng C 28:872-75 CrossRef
  6. Adanyi N, Szabo EE, Varadi M (1999) Multi-enzyme biosensors with amperometric detection for determination of lactose in milk and dairy products. Eur Food Res Technol 209:220-26 CrossRef
  7. Eshkenazi I, Maltz E, Zion B, Rishpon J (2000) A three-cascaded-enzymes biosensor to determine lactose concentration in raw milk. J Dairy Sci 83:1939-945 CrossRef
  8. Ferreira LS, De Souza MB, Trierweiler JO, Hitzmann B, Folly ROM (2003) Analysis of experimental biosensor/FIA lactose measurements. Braz J Chem Eng 20:7-3 CrossRef
  9. Ferreira LS, Trierweiler JO, De Souza MB, Folly RO (2004) A lactose FIA-biosensor system for monitoring and process control. Braz J Chem Eng 21:307-15 CrossRef
  10. Stoica L, Ludwig R, Haltrich D, Gorton L (2006) Third-generation biosensor for lactose based on newly discovered cellobiose dehydrogenase. Anal Chem 78(2):393-98 CrossRef
  11. Safina G, Ludwig R, Gorton L (2010) A simple and sensitive method for lactose detection based on direct electron transfer between immobilised cellobiose dehydrogenase and screen-printed carbon electrodes. Electrochim Acta 55:7690-695 CrossRef
  12. Ludwig R, Harreither W, Tasca F, Gorton L (2010) Cellobiose dehydrogenase: a versatile catalyst for electrochemical applications. Chemphyschem 11:2674-697 CrossRef
  13. Zamocky M, Ludwig R, Peterbauer C, Hallberg BM, Divne C, Nicholls P, Haltrich D (2006) Cellobiose dehydrogenase—a flavocytochrome from wood-degrading, phytopathogenic and saprotropic fungi. Curr Protein Pept Sci 7:255-80 CrossRef
  14. Beeson WT, Phillips CM, Cate JHD, Marletta MA (2012) Oxidative cleavage of cellulose by fungal copper-dependent polysaccharide monooxygenases. J Am Chem Soc 134:890-92 CrossRef
  15. Harreither W, Sygmund C, Augustin M, Narciso M, Rabinovich ML, Gorton L, Haltrich D, Ludwig R (2011) Catalytic properties and classification of cellobiose dehydrogenases from ascomycetes. Appl Environ Microbiol 77:1804-815 CrossRef
  16. Langston JA, Shaghasi T, Abbate E, Xu F, Vlasenko E, Sweeney MD (2011) Oxidoreductive cellulose depolymerization by the enzymes cellobiose dehydrogenase and glycoside hydrolase 61. Appl Environ Microbiol 77:7007-015 CrossRef
  17. Li X, Beeson WT, Phillips CM, Marletta MA, Cate JHD (2012) Structural basis for substrate targeting and catalysis by fungal polysaccharide monooxygenases. Structure 20:1051-061 CrossRef
  18. Phillips CM, Beeson WT, Cate JH, Marletta MA (2011) Cellobiose dehydrogenase and a copper-dependent polysaccharide monooxygenase potentiate cellulose degradation by / Neurospora crassa. ACS Chem Biol 6:1399-406 CrossRef
  19. Harreither W, Nicholls P, Sygmund C, Gorton L, Ludwig R (2012) Investigation of the pH-dependent electron transfer mechanism of ascomycetous class II cellobiose dehydrogenases on electrodes. Langmuir 28:6714-723 CrossRef
  20. Zafar MN, Safina G, Ludwig R, Gorton L (2012) Characteristics of third-generation glucose biosensors based on / Corynascus thermophilus cellobiose dehydrogenase immobilized on commercially available screen-printed electrodes working under physiological conditions. Anal Biochem 425(1):36-2 CrossRef
  21. Chekin F, Leiva N, Raoof JB, Gorton L, Bulow L (2010) Direct electrochemistry and bioelectrocatalysis of a class II non-symbiotic plant haemoglobin immobilised on screen-printed carbon electrodes. Anal Bioanal Chem 398:1643-649 CrossRef
  22. Schuhmann W, Wohlschkiger H, Huber J, Schmidt HL, Stadler H (1995) Development of an extremely flexible automatic analyzer with integrated biosensors for on-line control of fermentation processes. Anal Chim Acta 315:113-22 CrossRef
  23. Niculescu M, Erichsen T, Sukharev V, Zoltan K, Cs?regi E, Schuhmann W (2002) Quinohemoprotein alcohol dehydrogenase-based reagentless amperometric biosensor for ethanol monitoring during wine fermentation. Anal Chim Acta 463:39-1 CrossRef
  
• 作者单位：Nick Glithero (1)
  Claire Clark (1)
  Lo Gorton (2)
  Wolfgang Schuhmann (3)
  Neil Pasco (1)
  
  1. Lincoln Ventures Limited, PO Box 133, Lincoln, Christchurch, 7640, New Zealand
  2. Department of Analytical Chemistry/Biochemistry and Structural Biology, Lund University, PO Box 124, 22100, Lund, Sweden
  3. Analytical Chemistry Department, Bochum University, 44801, Bochum, Germany
  
• ISSN：1618-2650

文摘

Environmental and process control applications have needs for sensors that operate continuously or repeatedly, making them applicable to batch measurement and flowing product stream measurement. Additionally, for lactose monitoring in dairy-processing plants, the sensors must have sufficient flexibility to handle a wide range of substrate concentration and be resilient to withstand wide pH excursions brought about by frequent exposure to clean-in-place chemicals that happen without any warning. This paper describes the development and trialling of an at-line lactose biosensor that meets the needs of the dairy industry for loss monitoring of lactose in dairy-processing plants by the combination of a third-generation enzyme biosensor with a sequential injection analyser. Results, both from grab sample analysis and an at-line factory prototype, are shown from their operation when installed at a Fonterra dairy factory (New Zealand) during the 2011-012 season. Previous sensor fabrication methods were converted to a single-step process, and the flow-through cell was adapted to bubble-free operation. The lactose concentration in wastewater-processing streams was successfully monitored by taking and analysing samples every 2-?min, semi-continuously, for 3?months by an unskilled operator. The Fonterra site flushes approximately 100-00,000?L of wastewater per hour from its lactose plant. In the 2011-012 season, the daily mean lactose content of this wastewater varied significantly, from 0.0 to 8.0?% w/v (0-33,712?μM) and equated to substantial total losses of lactose over a 6-month period. These lactose losses represent lost saleable or useable product.