No association between polymorphism in the vascular endothelial growth factor gene at position?60 and sporadic prostate cancer in the Turkish population

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• 作者：Ilke H. Onen ; Ece Konac ; Muzaffer Eroglu ; Cagri Guneri ; Hasan Biri and Abdullah Ekmekci
• 关键词：Genotyping ; Prostate cancer ; Single nucleotide polymorphism ; Vascular endothelial growth factor
• 刊名：Molecular Biology Reports
• 出版年：2008
• 出版时间：March, 2008
• 年：2008
• 卷：35
• 期：1
• 页码：17-22
• 全文大小：194.7 KB

文摘

The development and progression of prostate cancer (PCa) has biologically and genetically remained a mystery. A man’s risk of developing PCa is influenced by both genetic and environmental factors. Angiogenic cytokines like vascular endothelial growth factor (VEGF) play a pivotal role in tumor angiogenesis. Single nucleotide polymorphisms in angiogenesis-dependent genes affect the sensibility of cancer development and progression. Therefore, we hypothesized a potential association between DNA sequence variations in VEGF −460 gene region and sporadic PCa patients in the Turkish population. 133 sporadic PCa patients and 157 healthy controls were studied. Genotypes were determined by polymerase chain reaction (PCR) and restriction fragment length polymorphism (RFLP) analysis. The distribution of genotype and allele frequencies of the polymorphism did not yield a statistically significant difference between patients and controls (P > 0.05). Furthermore, classification of patients by tumor-lymph nodes-metastasis (TNM), Gleason Scores (GS) and serum prostate-specific antigen (PSA) levels did not show significant differences among the VEGF −460 C>T genotypes (P > 0.05). This is the first demonstration showing that the VEGF −460 C>T polymorphism in men is not associated with sporadic PCa in the Turkish population.