Direct electrochemistry of hemoglobin in a renewable mesoporous carbon ceramic electrode: a new kind of hydrogen peroxide biosensor

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• 作者：Biuck Habibi ; Mojtaba Jahanbakhshi
• 关键词：Hemoglobin ; Hydrogen peroxide ; Biosensor ; Mesoporous carbon ; Sol–gel
• 刊名：Microchimica Acta
• 出版年：2015
• 出版时间：April 2015
• 年：2015
• 卷：182
• 期：5-6
• 页码：957-963
• 全文大小：469 KB
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• 刊物类别：Chemistry and Materials Science
• 刊物主题：Chemistry
  Analytical Chemistry
  Inorganic Chemistry
  Physical Chemistry
  Characterization and Evaluation Materials
  Monitoring, Environmental Analysis and Environmental Ecotoxicology
  
• 出版者：Springer Wien
• ISSN：1436-5073

文摘

A sol–gel derived renewable biosensor was prepared by entrapping hemoglobin (Hb) in a mesoporous carbon ceramic electrode (MCCE) in order to study the direct electron transfer of Hb and to fabricate a sensitive biosensor for hydrogen peroxide (H2O2). The Hb in the MCCE retained its original structure and still displayed good electrochemical activity in showing a pair of well-defined redox peaks as a result of direct electron transfer between Hb and the electrode surface. The MCCE possesses good biocompatibility, renewability, uniformity, and is well suited for immobilization of enzymes. The immobilized Hb was found to display good electrocatalytic activity for the reduction of H2O2, and this resulted in the design of an amperometric biosensor working at ?.3?V and capable of sensing H2O2 with a detection limit of 0.4?μM (at a S/N of 3). The catalytic reduction current is proportional to the concentration of H2O2 in the 1.0 to 220.0?μM range. This work demonstrates MCCE to be a well suited and easily accessible substrate for engineering biosensors with various kinds of potential applications. Graphical Abstract Electroreduction of hydrogen peroxide with a mesoporous carbon ceramic electrode containing immobilized hemoglobin.