Puerarin Protects Hippocampal Neurons Against Cell Death in Pilocarpine-Induced Seizures Through Antioxidant and Anti-Apoptotic Mechanisms

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• 作者：Nanchang Xie (1)
  Cui Wang (2)
  Yajun Lian (1)
  Chuanjie Wu (1)
  Haifeng Zhang (1)
  Qian Zhang (1)
  
• 关键词：Puerarin ; Epilepsy ; Mitochondria ; Apoptosis ; Oxidative stress
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2014
• 出版时间：November 2014
• 年：2014
• 卷：34
• 期：8
• 页码：1175-1182
• 全文大小：1,179 KB
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• 作者单位：Nanchang Xie (1)
  Cui Wang (2)
  Yajun Lian (1)
  Chuanjie Wu (1)
  Haifeng Zhang (1)
  Qian Zhang (1)
  
  1. Department of Neurology, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, 450052, China
  2. Department of Internal Medicine, The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China
  
• ISSN：1573-6830

文摘

Puerarin extracted from Radix puerariae has been shown to exert neuroprotective effects. However, it is still not known whether puerarin protects hippocampal neurons against cell death in pilocarpine-induced seizures. In this study, we found that pretreatment with puerarin significantly attenuated the neuronal death in the hippocampus of rats with pilocarpine-induced epilepsy. In addition, puerarin decreased the level of seizure-induced reactive oxygen species in mitochondria isolated from the rat hippocampi. Terminal deoxyuridine triphosphate nick-end labeling staining showed that puerarin exerted an anti-apoptotic effect on the neurons in the epileptic hippocampus. Western blot analysis showed that puerarin treatment significantly decreased the expression of Bax and increased the expression of Bcl-2. Moreover, puerarin treatment restored the altered mitochondrial membrane potential and cytochrome c release from the mitochondria in the epileptic hippocampi. Altogether, the findings of this study suggest that puerarin exerts a therapeutic effect on epilepsy-induced brain injury through antioxidant and anti-apoptotic mechanisms.