Effects of Hypothermia on S100B and Glial Fibrillary Acidic Protein in Asphyxia Rats After Cardiopulmonary Resuscitation

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• 作者：Sha Liu ; Yibing Zhang ; Yong Zhao ; Haifeng Cui…
• 关键词：Cardiopulmonary resuscitation ; Hypothermia ; Brain protection ; S100B ; GFAP
• 刊名：Cell Biochemistry and Biophysics
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：71
• 期：1
• 页码：401-406
• 全文大小：505 KB
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• 刊物主题：Biochemistry, general; Pharmacology/Toxicology; Biotechnology; Cell Biology; Biophysics and Biological Physics;
• 出版者：Springer US
• ISSN：1559-0283

文摘

The aim of the study was to investigate the effects of hypothermia on S100B and glial fibrillary acidic protein (GFAP) in serum and hippocampus CA1 area in asphyxiated rats after cardiopulmonary resuscitation (CPR). A total of 100 SD rats were designated into four groups: group A, sham operation group; group B, rats received conventional resuscitation; group C, rats received conventional resuscitation and hypothermia at cardiac arrest; group D, rats received conventional resuscitation and hypothermia at 30?min after restoration of spontaneous circulation (ROSC). Rats were then killed by cardiac arrest at 2 and 4?h after ROSC; brain tissue was taken to observe dynamic changes of S100B and GFAP in serum and hippocampus CA1 area. Following ROSC, S100B levels increased from 2 to 4?h in group B, C, and D. In addition, S100B in serum and hippocampus CA1 area was all significantly increased at different time points compared with group A (P?P?>?0.05). Moreover, S100B in serum at 4?h after ROSC was significantly decreased (P?P?GFAP was also examined. GFAP level in hippocampus CA1 area was significantly decreased in group B, C, and D at 4?h after ROSC compared with group A (P?GFAP were expressed in rat serum and hippocampus CA2 area at early stage after ROSC, which can be used as sensitive markers for brain injury diagnosis and prognosis prediction. Hypothermia is also shown to reduce brain injury after CPR.