Chromosome elimination and introgression following somatic hybridization between bread wheat and other grass species

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• 作者：Haifeng Cui (1) (2)
  Yang Sun (1)
  Jingyao Deng (1)
  Minqin Wang (1)
  Guangmin Xia (1)
  
• 关键词：Triticum aestivum L. ; Somatic hybridization ; Chromosome elimination ; Chromosome introgression ; Micronuclei ; Radiation dosage ; Phylogenetical relationship
• 刊名：Plant Cell, Tissue and Organ Culture
• 出版年：2015
• 出版时间：January 2015
• 年：2015
• 卷：120
• 期：1
• 页码：203-210
• 全文大小：1,545 KB
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• 作者单位：Haifeng Cui (1) (2)
  Yang Sun (1)
  Jingyao Deng (1)
  Minqin Wang (1)
  Guangmin Xia (1)
  
  1. The Key Laboratory of Plant Cell Engineering and Germplasm Innovation, Ministry of Education, School of Life Sciences, Shandong University, Jinan, 250100, People’s Republic of China
  2. Zhejiang Provincial Key Laboratory of Biometrology and Inspection and Quarantine, College of Life Sciences, China Jiliang University, Hangzhou, 310018, People’s Republic of China
  
• ISSN：1573-5044

文摘

The timing and influencing factor of donor chromosome elimination and introgression in somatic hybrids involving bread wheat (Triticum aestivum L.) was investigated by sampling fusion products made between bread wheat and four different donor species. Genomic in situ hybridization experiments showed that both events in the main occurred within 12-5?days of the fusion. Over 70?% of the elimination events had taken place before the first mitotic interphase. Pre-treating the donor protoplasts by either γ or UV irradiation showed that the severity of exposure affected the likelihood of chromosome elimination and introgression. More donor chromosome elimination and less introgression occurred when donors were phylogenetically more distant, such as annual meadow grass (Poaannua) or maize (Zea mays), than phylogenetically closer donors such as tall wheatgrass [Agropyron elongatum (Thinopyrum elongatum)] or Haynaldia villosa. The interaction effect between donor species and irradiation time significantly affected the chromatin behaviors during somatic hybridization. Besides, the polyploid A. elongatum behaved differently from diploid H. villosa, even though these two species are phylogenetically equidistant from wheat.