Identity of M2A (D2-40) antigen and gp36 (Aggrus, T1A-2, podoplanin) in human developing testis, testicular carcinoma in situ and germ-cell tumours
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- 作者：Si Brask Sonne ; Amy S. Herlihy ; Christina E. Hoei-Hansen ; John E. Nielsen ; Kristian Almstrup ; Niels E. Skakkebaek ; Alexander Marks ; Henrik Leffers and Ewa Rajpert-De Meyts
- 关键词：M2A ; PDPN ; Intratubular germ ; cell neoplasia (IGCN) ; Testicular germ ; cell neoplasm ; Testicular development ; Sertoli cells
- 刊名：Virchows Archiv
- 出版年：2006
- 出版时间：August, 2006
- 年：2006
- 卷：449
- 期：2
- 页码：200-206
- 全文大小：344 KB
- 刊物类别：Medicine
- 刊物主题：Medicine & Public Health
Pathology - 出版者：Springer Berlin / Heidelberg
- ISSN：1432-2307
文摘
Testicular germ-cell tumours of young adults are derived from a pre-invasive intratubular lesion, carcinoma in situ (CIS). In a recent genome-wide gene expression screening using cDNA microarrays, we found PDPN over-expressed in CIS compared to normal adult testis. PDPN encodes podoplanin (Aggrus, human gp36, T1A-2), a transmembrane glycoprotein expressed in lymphatic endothelium and various solid tumours. To examine a potential role for PDPN in testicular neoplasms and during testicular development, we investigated its expression pattern during the development of human testis and in a series of testicular CIS, gonadoblastoma and overt germ-cell tumours. We established by RT-PCR and by immunohistochemistry with a gp36 antibody that PDPN mRNA and the protein product were expressed in testes with germ-cell neoplasms but not in the normal adult testis. We also found gp36 expression in early foetal gonocytes and immature Sertoli cells, similar to the expression pattern of M2A antigen, a previously identified marker for CIS and seminoma. This reinforced our previous proposal that M2A (D2-40) antigen was identical to gp36 (podoplanin, Aggrus, T1A-2). Our findings also suggest that podoplanin has a function in developing testis, most likely at the level of cell–cell interactions among pre-meiotic germ cells and immature Sertoli cells.