Adenovirus-Mediated Coexpression of DCX and SPARC Radiosensitizes Human Malignant Glioma Cells

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• 作者：Yuanyuan Xu (1)
  Lei Yang (1)
  Xin Jiang (1)
  Jiahua Yu (1)
  Jicheng Yang (2)
  Haowen Zhang (1)
  Guomei Tai (1)
  Xiaopeng Yuan (1)
  Fenju Liu (1)
  
• 关键词：DCX ; SPARC ; Glioma ; Radiosensitivity ; Adenovirus
• 刊名：Cellular and Molecular Neurobiology
• 出版年：2013
• 出版时间：October 2013
• 年：2013
• 卷：33
• 期：7
• 页码：965-971
• 全文大小：633KB
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• 作者单位：Yuanyuan Xu (1)
  Lei Yang (1)
  Xin Jiang (1)
  Jiahua Yu (1)
  Jicheng Yang (2)
  Haowen Zhang (1)
  Guomei Tai (1)
  Xiaopeng Yuan (1)
  Fenju Liu (1)
  
  1. Department of Radiobiology, School of Radiation Medicine and Protection, Medical College of Soochow University, School for Radiological and Interdisciplinary Sciences, Soochow University, No. 199 Ren鈥檃i Street, Suzhou, 215123, China
  2. Cell and Molecular Biology Institute, Medical College of Soochow University, Suzhou, 215123, China
  
• ISSN：1573-6830

文摘

This study is designed to examine the radiosensitizing effects of coexpression of doublecortin (DCX) and secreted protein and rich in cysteine (SPARC). Previously, we showed that downregulation of SPARC by small interfering RNA increased radioresistance of U-87MG glioma cells. Therefore, overexpression of SPARC might increase radiosensitivity of glioma cells. But SPARC has been shown to promote glioma cell invasion both in vitro and vivo. In order to radiosensitize glioma cells without stimulating invasion, we chose DCX, which is a well-characterized anti-tumor gene, to coexpress with SPARC. An adenovirus-mediated double gene expression system was constructed and applied to U251 and A172 glioma cell lines. Our data showed that coexpression of DCX and SPARC collaboratively diminished radioresistance of glioma cells, interfered with cell cycle turnover and increased irradiation-induced apoptosis. In addition, transwell assay revealed that coexpression was able to counteract the invasion-promoting effects of SPARC, and even inhibited intrinsic invasion, evidenced by less invading cells in double gene overexpressed group than that of control adenovirus-treated group. In conclusion, genetic engineering combining two or more genes might be a more effective method to overcome radioresistance of glioma cells.