A green fluorescent protein-engineered haploid inducer line facilitates haploid mutant screens and doubled haploid breeding in maize

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• 作者：Weichang Yu ; James A. Birchler
• 关键词：Haploid ; Doubled haploid (DH) ; Green fluorescent protein (GFP) ; Chromosome doubling ; Maize
• 刊名：Molecular Breeding
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：36
• 期：1
• 全文大小：1,739 KB
• 参考文献：401 Research Group, Institute of Genetics, Academia Sinia (1975) Primary study on induction of pollen plants of Zea mays. Acta Genet Sin 2:143–145 (in Chinese)
  Armstrong CL, Green CE, Phillips RL (1991) Development and availability of germplasm with high type II culture formation response. Maize Genet Coop Newsletter 65:92–93
  Barret P, Brinkman M, Dufour P, Murigneux A, Beckert M (2004) Identification of candidate genes for in vitro androgenesis induction in maize. Theor Appl Genet 109:1660–1668CrossRef PubMed
  Barret P, Brinkmann M, Beckert M (2008) A major locus expressed in the male gametophyte with incomplete penetrance is responsible for in situ gynogenesis in maize. Theor Appl Genet 117:581–594CrossRef PubMed
  Beckert M (1994) Advantages and disadvantages of the use of in vitro/in situ produced DH maize plants. In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 25. Springer, Berlin, pp 201–213
  Bevan M (1984) Binary Agrobacterium vectors for plant transformation. Nucleic Acids Res 12:8711–8721PubMedCentral CrossRef PubMed
  Burr FA, Burr B, Scheffler BE, Blewitt M, Wienand U, Matz EC (1996) The maize repressor-like gene intensifier1 shares homology with the r1/b1 multigene family of transcription factors and exhibits missplicing. Plant Cell 8:1249–1259PubMedCentral CrossRef PubMed
  Chaikam V, Mahuku G (2012) Chromosome doubling of maternal haploids. In: Prasanna BM, Chaikam V, Mahuku G (eds) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico, pp 24–29
  Chaikam V, Nair SK, Babu R, Martinez L, Tejomurtula J, Boddupalli PM (2015) Analysis of effectiveness of R1-nj anthocyanin marker for in vivo haploid identification in maize and molecular markers for predicting the inhibition of R1-nj expression. Theor Appl Genet 128:159–171CrossRef PubMed
  Chang MT, Coe EH (2009) Doubled haploids. In: Kriz AL, Larkins BA (eds) Biotechnology in agriculture and forestry. Springer, Berlin, pp 127–142
  Chase SS (1949) Monoploid frequencies in a commercial double cross hybrid maize, and in its component single cross hybrids and inbred lines. Genetics 34:328–332PubMedCentral PubMed
  Chen SJ, Song TM (2003) Identification haploid with high oil xenia effect in maize. Acta Agron Sin 29:587–590 (in Chinese)
  Chiu W, Niwa Y, Zeng W, Hirano T, Kobayashi H, Sheen J (1996) Engineered GFP as a vital reporter in plants. Curr Biol 6:325–330CrossRef PubMed
  Coe EH (1959) A line of maize with high haploid frequency. Am Nat 93:381–382CrossRef
  Cormack BP, Valdivia RH, Falkow S (1996) FACS-optimized mutants of the green fluorescent protein (GFP). Gene 173:33–38CrossRef PubMed
  Datla RSS, Bekkaoui F, Hammerlindl JK, Pilate G, Dunstan DI, Crosby WL (1993) Improved high-level constitutive foreign gene expression in plants using an AMV RNA4 untranslated leader sequence. Plant Sci 94:139–149CrossRef
  Della Vedova CB, Lorbiecke R, Kirsch H, Schulte MB, Scheets K, Borchert LM, Scheffler BE, Wienand U, Cone KC, Birchler JA (2005) The dominant inhibitory chalcone synthase allele C2-Idf (inhibitor diffuse) from Zea mays (L.) acts via an endogenous RNA silencing mechanism. Genetics 170:1989–2002PubMedCentral CrossRef PubMed
  Eder J, Chalyk S (2002) In vivo haploid induction in maize. Theor Appl Genet 104:703–708CrossRef PubMed
  Frame BR, Shou H, Chikwamba RK, Zhang Z, Xiang C, Fonger TM, Pegg SE, Li B, Nettleton DS, Pei D, Wang K (2002) Agrobacterium tumefaciens-mediated transformation of maize embryos using a standard binary vector system. Plant Physiol 129:13–22PubMedCentral CrossRef PubMed
  Geiger HH, Gordillo GA (2009) Doubled haploids in hybrid maize breeding. Maydica 54:485–499
  Gross SM, Hollick JB (2007) Multiple trans-sensing interactions affect meiotically heritable epigenetic states at the maize pl1 locus. Genetics 176:829–839PubMedCentral CrossRef PubMed
  Hood EE, Helmer GL, Fraley RT, Chilton MD (1986) The hypervirulence of Agrobacterium tumefaciens A281 is encoded in a region of pTiBo542 outside of T-DNA. J Bacteriol 168:1291–1301PubMedCentral PubMed
  Kaeppler HF, Carlson AR, Menon GK (2001) Routine utilization of green fluorescent proteins as a visual selectable marker for cereal transformation. In Vitro Cell Dev Biol Plant 37:120–126CrossRef
  Kebede AZ, Dhillon BS, Schipprack W, Araus JL, Banziger M, Semagan K, Alvarado G, Melchinger AE (2011) Effect of source germplasm and season on the in vivo haploid induction rate in tropical maize. Euphytica 180:219–226CrossRef
  Kuo CS, Sun AC, Wang YY, Gui YL, Gu SR, Miao SH (1978) Studies on induction of pollen plants and androgenesis in maize. Acta Bot Sin 20:204–209 (in Chinese)
  Lashermes P, Beckert M (1988) Genetic control of maternal haploidy in maize (Zea mays L.) and selection of haploid inducing lines. Theor Appl Genet 76:405–410PubMed
  Li L, Xu X, Jin W, Chen S (2009) Morphological and molecular evidences for DNA introgression in haploid induction via a high oil inducer CAUHOI in maize. Planta 230:367–376CrossRef PubMed
  Nanda DK, Chase SS (1966) An embryo marker for detecting monopoids of maize (Zea mays L.). Crop Sci 6:213–215CrossRef
  Niedz RP, McKendree WL, Shatters JRRG (2003) Electroporation of embryogenic protoplasts of sweet orange (Citrus sinensis (L.) Osbeck) and regeneration of transformed plants. In Vitro Cell Dev Biol Plant 39:586–594CrossRef
  Paz-Ares J, Ghosal D, Saedler H (1990) Molecular analysis of the C1-I allele from Zea mays: a dominant mutant of the regulatory C1 locus. EMBO J 9:315–321PubMedCentral PubMed
  Prasanna BM, Chaikam V, Mahuku G (2012) Doubled haploid technology in maize breeding: theory and practice. CIMMYT, Mexico
  Prigge V, Sanchez C, Dhillon BS, Schipprack W, Araus JL, Banziger M, Melchinger AE (2011) Doubled haploids in tropical maize: I. Effects of inducers and source germplasm on in vitro haploid induction rates. Crop Sci 51:1498–1506CrossRef
  Prigge V, Xu X, Li L, Babu R, Chen S, Atlin GN, Melchinger AE (2012) New insights into the genetics of in vivo induction of maternal haploids, the backbone of doubled haploid technology in maize. Genetics 190:781–793PubMedCentral CrossRef PubMed
  Raizada MN, Nan GL, Walbot V (2001) Somatic and germinal mobility of the RescueMu transposon in transgenic maize. Plant Cell 13:1587–1608PubMedCentral CrossRef PubMed
  Röber FK, Gordillo GA, Geiger HH (2005) In vivo haploid induction in maize—performance of new inducers and significance of doubled haploid lines in hybrid breeding. Maydica 50:275–283
  Rotarenco VA, Kirtoca IH, Jacota AG (2007) The possibility of identifying kernels with haploid embryos using oil content. Maize Genet Coop Newsletter 81:11
  Rotarenco VA, Dicu G, State D, Fuia S (2010) New inducers of maternal haploids in maize. Maize Genet Coop Newsletter 84:15
  Sarkar KR, Pandey A, Gayen P, Mandan JK, Kumar R, Sachan JKS (1994) Stabilization of high haploid inducer lines. Maize Genet Coop Newsletter 68:64–65
  Shimomura O, Johnson FH, Saiga Y (1962) Extraction, purification and properties of aequorin, a bioluminescent protein from the luminous hydromedusan, Aequorea. J Cell Comp Physiol 59:223–239CrossRef PubMed
  Stuitje AR, Verbree EC, van der Linden KH, Mietkiewska EM, Nap JP, Kneppers TJ (2003) Seed-expressed fluorescent proteins as versatile tools for easy (co)transformation and high-throughput functional genomics in Arabidopsis. Plant Biotechnol J 1:301–309CrossRef PubMed
  Tracy WF (1997) History, genetics, and breeding of supersweet (shrunken2) sweet corn. In: Janick J (ed) Plant breeding reviews, vol 14. John Wiley & Sons, New York, pp 189–236
  Tyrnov VS, Zavalishina AN (1984) Inducing high frequency of matroclinal haploids in maize. Dokl Akad Nauk SSSR 276:735–738 (in Russian)
  Weigel D, Glazebrook J (2006) Transformation of Agrobacterium using the freeze-thaw method. CSH Protoc 2006(7). doi:10.​1101/​pdb.​prot4666
  Zhang Z, Qiu F, Liu Y, Ma K, Li Z, Xu S (2008) Chromosome elimination and in vivo haploid production induced by Stock 6-derived inducer line in maize (Zea mays L.). Plant Cell Rep 27:1851–1860CrossRef PubMed
  Zhao X, Xu X, Xie H, Chen S, Jin W (2013) Fertilization and uniparental chromosome elimination during crosses with maize haploid inducers. Plant Physiol 163:721–731PubMedCentral CrossRef PubMed
  
• 作者单位：Weichang Yu (1)
  James A. Birchler (2)
  
  1. Shenzhen Research Institute, Chinese University of Hong Kong, Shenzhen, China
  2. Division of Biological Sciences, University of Missouri, Columbia, MO, 65211, USA
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Plant Sciences
  
• 出版者：Springer Netherlands
• ISSN：1572-9788

文摘

Doubled haploid (DH) technology is a useful tool for maize breeding and gene discovery. The production of a large number of DH lines has been possible recently with the development of various in vivo haploid inducer lines based on the discovery of the initial stock 6. Most of the haploid induction systems use the R1-navajo (R1-nj) gene whose expression in the kernel produces diploid kernels with colored aleurone crowns and scutella and haploid kernels with colored aleurone crowns but colorless scutella. However, the R1-nj gene expression depends on various genetic and environmental factors, which can mask the typical R1-nj phenotype. To solve this problem, we introduced a dominant green fluorescent protein (GFP) marker gene into a maize haploid inducer, RWS, to generate a RWS-GFP inducer. This line allows the identification of haploids in the early germination stage by visualizing the GFP expression of germinated kernels. Germinated diploid seeds will produce GFP fluorescence in emerged radicles and coleoptiles, but haploids will be GFP negative because of the lack of paternal GFP gene during hybridization with the haploid inducer. We demonstrated that this system can be used to screen haploid mutants and to produce haploids from various commercial sweet corn hybrids, which have a genetic background that prevents haploid identification by other systems. Haploid plants were treated with colchicine to produce DH plants. The GFP-engineered haploid inducer line will be a powerful tool in maize genetic studies and DH breeding.