An Anti-Tumor Peptide from Musca domestica Pupae (MATP) Induces Apoptosis in Human Liver Cancer Cells HepG2 Cells Through a ROS-JNK Pathway

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• 作者：Dai Cheng ; Meng Meng ; Xinyu Zhang…
• 关键词：Anti ; tumor peptide ; Apoptosis ; ROS ; JNK ; Caspase
• 刊名：International Journal of Peptide Research and Therapeutics
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：23
• 期：1
• 页码：101-109
• 全文大小：
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biochemistry, general; Animal Anatomy / Morphology / Histology; Polymer Sciences; Pharmaceutical Sciences/Technology; Pharmacology/Toxicology; Molecular Medicine;
• 出版者：Springer Netherlands
• ISSN：1573-3904
• 卷排序：23

文摘

An anti-tumor peptide from Musca domestica pupae (MATP) inhibited proliferation of human liver cancer cells HepG2 in a dose-dependent manner. The results of morphology observation indicated that MATP inducing HepG2 cells apoptosis based on the typical apoptotic morphological changes. Flow cytometric analysis demonstrated that MATP caused apoptosis of HepG2 cells through cells arrested at S phase (from 14.26 to 54.38 %) and the apoptotic rates significantly increased (from 1.34 to 25.20 %). The laser scanning confocal microscopy results showed that the generation of intracellular reactive oxygen species (ROS) was increased and the Western blot results revealed that ROS induced a sustained activation of phosphorylated-JNK. Simultaneously, the apoptosis induced by MATP was reversed by NAC (ROS inhibitor) and SP600125 (JNK inhibitor). These results proved that ROS/JNK participated in apoptosis of HepG2 treated with MATP. Moreover, Bax-to-Bcl-2 expression ratio was increased by the activation of phosphorylated-JNK. The release of Cytochrome c from mitochondria which arose the Caspases cascade enhanced by the increase of Bax-to-Bcl-2 expression ratio and intensified the expression of Caspase-9 and Caspase-3. Taken together, these findings suggest that the MATP induces apoptosis through a ROS/JNK-mediated Caspase pathway.