All-Trans Retinoic Acid Induces Expression of a Novel Intergenic Long Noncoding RNA in Adult rat Primary Hippocampal Neurons

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• 作者：Sukhleen Kour ; Pramod C. Rath
• 关键词：Long noncoding RNA ; Primary hippocampal neurons ; Retinoic acid ; Fluorescence in ; situ RNA hybridization ; Actinomycin D
• 刊名：Journal of Molecular Neuroscience
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：58
• 期：2
• 页码：266-276
• 全文大小：1,897 KB
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• 作者单位：Sukhleen Kour (1)
  Pramod C. Rath (1)
  
  1. Molecular Biology Laboratory, School of life Sciences, Jawaharlal Nehru University, New Delhi, -110067, India
  
• 刊物主题：Neurosciences; Neurochemistry; Cell Biology; Proteomics; Neurology;
• 出版者：Springer US
• ISSN：1559-1166

文摘

Around 90 % of the mammalian genome undergoes pervasive transcription into various types of small and long regulatory noncoding RNAs, whereas only ∼1.5 % codes for proteins. Long noncoding RNAs (lncRNAs) constitute diverse classes of sense- and antisense transcripts that are abundantly expressed in the mammalian central nervous system (CNS) in cell type- and developmental stage-specific manners. They are implicated in brain development, differentiation, neuronal plasticity, and other cognitive functions. Mammalian brain requires the vitamin A metabolite all-trans retinoic acid (atRA) for its normal development, differentiation, and cell-fate determination. However, its role in adult brain function is less understood. Here, we report atRA-mediated transcriptional upregulation of endogenous expression of a novel long intergenic noncoding RNA-rat brain expressed (LINC-RBE) in cultured primary hippocampal neurons from adult rat. We have previously reported LINC-RBE as an intergenic, simple repeat sequence containing lncRNA highly expressed in the rat brain. This is a first-time report of involvement of atRA in transcriptional upregulation of lncRNA expression in rat hippocampal neurons. Therefore, it may be involved in regulation of brain function and disease. Keywords Long noncoding RNA Primary hippocampal neurons Retinoic acid Fluorescence in-situ RNA hybridization Actinomycin D