TRPM7 Regulates Axonal Outgrowth and Maturation of Primary Hippocampal Neurons

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• 作者：Ekaterina Turlova ; Christine Y. J. Bae ; Marielle Deurloo…
• 关键词：TRPM7 ; Ion channel ; Neurite outgrowth ; Neuronal maturation ; Axonal development
• 刊名：Molecular Neurobiology
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：53
• 期：1
• 页码：595-610
• 全文大小：10,960 KB
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• 作者单位：Ekaterina Turlova (1) (2)
  Christine Y. J. Bae (1) (2)
  Marielle Deurloo (2)
  Wenliang Chen (1) (2)
  Andrew Barszczyk (2)
  F. David Horgen (5)
  Andrea Fleig (6) (7)
  Zhong-Ping Feng (2)
  Hong-Shuo Sun (1) (2) (3) (4)
  
  1. Department of Surgery, Faculty of Medicine, University of Toronto, 1132 Medical Sciences Building, 1 King’s College Circle, Toronto, ON, M5S 1A8, Canada
  2. Department of Physiology, Faculty of Medicine, University of Toronto, 3306 Medical Sciences Building, 1 King’s College Circle, Toronto, ON, M5S 1A8, Canada
  5. College of Natural and Computational Sciences, Hawaii Pacific University, Kaneohe, HI, 96744, USA
  6. Center for Biomedical Research, The Queen’s Medical Center, Honolulu, HI, 96720, USA
  7. University of Hawaii Cancer Center and John A. Burns School of Medicine, Honolulu, HI, 96720, USA
  3. Department of Pharmacology & Toxicology, Faculty of Medicine, University of Toronto, 1 King’s College Circle, Toronto, ON, M5S 1A8, Canada
  4. Institute of Medical Science, Faculty of Medicine, University of Toronto, 1 King’s College Circle, Toronto, ON, M5S 1A8, Canada
  
• 刊物主题：Neurosciences; Neurobiology; Cell Biology; Neurology;
• 出版者：Springer US
• ISSN：1559-1182

文摘

Transient receptor potential melastatin 7 (TRPM7) is a calcium-permeable divalent cation channel and mediates neuronal cell death under ischemic stresses. In this study, we investigated the contribution of TRPM7 to neuronal development in mouse primary hippocampal neurons. We demonstrated that TRPM7 channels are highly expressed in the tips of the growth cone. Either knockdown of TRPM7 with target-specific shRNA or blocking channel conductance by a specific blocker waixenicin A enhanced axonal outgrowth in culture. Blocking TRPM7 activity by waixenicin A reduced calcium influx and accelerated the polarization of the hippocampal neurons as characterized by the development of distinct axons and dendrites. Furthermore, TRPM7 coprecipitated and colocalized with F-actin and α-actinin-1 at the growth cone. We conclude that calcium influx through TRPM7 inhibits axonal outgrowth and maturation by regulating the F-actin and α-actinin-1 protein complex. Inhibition of TRPM7 channel promotes axonal outgrowth, suggesting its therapeutic potential in neurodegenerative disorders.