A homeopathic remedy from arnica, marigold, St. John’s wort and comfrey accelerates in vitro wound scratch closure of NIH 3T3 fibroblasts

详细信息    查看全文

• 作者：Katarina Hostanska (1)
  Matthias Rostock (1) (2)
  Joerg Melzer (1)
  Stephan Baumgartner (3) (4) (5)
  Reinhard Saller (1)
  
• 关键词：Wound healing ; 3T3 fibroblasts ; Homeopathic remedy ; Arnica ; Calendula ; Hypericum ; Symphytum
• 刊名：BMC Complementary and Alternative Medicine
• 出版年：2012
• 出版时间：December 2012
• 年：2012
• 卷：12
• 期：1
• 全文大小：1649KB
• 参考文献：1. Singer AJ, Clark RA: Cutaneous wound healing. / N Engl J Med 1999, 341:738-46. CrossRef
  2. De Fatima A, Modolo LV, Conegero Sanches AC, Porto RR: Wound healing agents: The role of natural and non-natural products in drug development. / Mini Rev Med Chem 2008, 8:879-88. CrossRef
  3. Gal P, Toporcer T, Grendel T, Vidova Z, Smetana K Jr, Dvorankova B, Gal T, Mozes S, Lenhardt L, Longauer F, Sabol M, Sabo J, Backor M: Effect of Atropa belladonna L. on skin wound healing: Biomechanical and histological study in rats and in vitro study in keratinocytes, 3T3 fibroblasts, and human umbilical vein endothelial cells. / Wound Repair Regen 2007, 17:378-86. CrossRef
  4. Goldman RS, de Freitas PCD, Oga S: Wound healing and analgesic effect of crude extracts of Symphytum officinale in rats. / Fitoterapia 1985, 66:323-29.
  5. Leu S, Havey J, White LE, Martin N, Yoo SS, Rademaker AW, Alam M: Accelerated resolution of laser-induced bruising with topical 20% arnica: a rater-blinded randomized controlled trial. / Br J Dermatol 2010, 163:557-63. CrossRef
  6. Chadran PK, Kuttan R: Effect of Calendula officinalis flower extract on acute phase proteins, antioxidant defense mechanism and granuloma formation during thermal burns. / J Cli Biochem Nutr 2008, 43:58-4. CrossRef
  7. Reuter J, Merfort I, Schempp CM: Botanicals in dermatology. An evidence-based review. / Am J Clin Dermatol 2010, 11:247-67.
  8. Fronza M, Heinzmann B, Hamburger M, Laufer S, Merfort I: Determination of the wound healing effect of Calendula extracts using the scratch assay with 3T3 fibroblasts. / J Ethnopharmacol 2009, 126:463-67. CrossRef
  9. Lavagna SM, Secci D, Chimenti P, Bonsignore L, Ottaviani A, Bizzari B: Efficacy of Hypericum and Calendula oils in the epithelial reconstruction of surgical wounds in childbirth with caesarean section. / Farmaco 2001, 56:451-53. CrossRef
  10. Dikmen M, Oztürk Y, Sagratini G, Ricciutelli M, Vittori S, Maggi F: Evaluation of the wound healing potentials of two subspecies of Hypericum perforatum on cultured NIH3T3 fibroblasts. / Phytother Res 2011, 25:208-14.
  11. Ernst E, Pittler MH: Efficacy of homeopathic arnica. / Arch Surg 1998, 133:1187-190. CrossRef
  12. Balzarini A, Felisi E, Martini A, De Conno F: Efficacy of homeopathic treatment of skin reactions during radiotherapy for breast cancer: a randomised, double-blind clinical trial. / Br Homeopathic J 2000, 89:8-2. CrossRef
  13. Macedo SB, Ferreira LR, Perrazzo LL, Tavarea-Carvalho JC: Antiinflammatory activity of Arnica montana 6cH. Preclinical study in animals. / Homeopathy 2004, 93:84-7. CrossRef
  14. Seeley BM, Denton AB, Ahn MS, Maas CS: Effect of homeopathic Arnica Montana on bruising in face-lifts: results of a randomized, double-blind, placebo-controlled clinical trials. / Arch Facial Plast Surg 2006, 8:54-9. CrossRef
  15. Karow JH, Abt HP, Fr?hling M, Ackermann H: Efficacy of Arnica montana D4 for healing of wounds after Hallux valgus surgery compared to diclofenac. / J Altern Complement Med 2008, 14:17-5. CrossRef
  16. Stevinson C, Devaraj VS, Fountain Barber A, Hawkins S, Ernst E: Homeopathic arnica for prevention of pain and bruising: randomized placebo-controlled trial in hand surgery. / J R Soc Med 2003, 96:60-4. CrossRef
  17. Kotlus SB, Heringr MD, Drydem MR: Evaluation of homeopathic Arnica montana for ecchymosis after upper blepharoplasty: a placebo-controlled, randomized, double-blind study. / Ophthal Plast Reconstr Surg 2010, 26:395-97. CrossRef
  18. Bresler A, Hawkins D, Raziog R, Abrahamse H: Effect of low level laser therapy and Calendula officinalis 3 CH on wound healing in human skin fibroblasts. / Ind J Res Hom 2008, 2:7-5.
  19. Liang CC, Park AY, Guan JL: In vitro scratch assay: a convenient and inexpensive method for analysis of a cell migration in vitro. / Nat Protoc 2007, 2:329-33. CrossRef
  20. German Homeopathic Pharmacopoiea Wissentschaftliche Verlagsgesellschaft mbH Stuttgart: MedPharm Scientific Publishers; 2010.
  21. Hostanska K, Melzer J, Amon A, Saller R: Suppression of interleukin (IL)-8 and human beta defensin-2 secretion in LPS-and /or IL-1?-stimulated epithelial A549 cell by a herbal formulation against respiratory infections (BNO 1030). / J Ethnopharmacol 2011, 134:228-33. CrossRef
  22. Hardonk MJ, Harms G: The use of 5-Bromodeoxyuridine in the study of cell proliferation. / Acta Histochemica 1990, Suppl.39:99-08.
  23. Hostanska K, Nisslein T, Freudenstein J, Reichling J, Saller R: Inhibitory effect of an isopropanolic extract of black cohosh on invasiveness of MDA-MB 231 human breast cancer cells. / In vivo 2007, 21:349-56.
  24. Selinummi J, Seppala J, Yli-Harja O, Puhakka JA: Software for quantification of labeled bacteria from digital microscope images by automated image analysis. / Biotechniques 2005, 39:859-63. CrossRef
  25. Fukaya K, Miyazaki M, Pu H, Katayama B, Inoue Y, Ohashi R, Nakamura C, Namba M: Pyruvate alleviates toxic effect of ethanol on cells in culture. / Arch Toxicol 1997, 71:651-54. CrossRef
  26. Gradl G, Faust D, Oesch F, Wieser RJ: Density-dependent regulation of cell growth by contactinhibin and the contactinibin receptor. / Curr Biol 1995, 5:526-35. CrossRef
  27. Graham MF, Diegelman RF, Cohen IK: An in vitro model of fibroplasia: simultaneous quantification of fibroblast proliferation, migration, and collagen synthesis. / Proc Soc Exp Biol Med 1984, 176:302-08.
  28. Lauffenburger DA, Horwitz AF: Cell migration: a physically integrated molecular process. / Cell 1996, 84:359-69. CrossRef
  29. Engstr?m W: Differential effects of epidermal growth factor (EGF) on cell locomotion and cell proliferation in a cloned human embryonal carcinoma-derived cell line in vitro. / J Cell Sci 1986, 86:47-5.
  30. Carmine TC: Effects of high potencies of tumor necrosis factor alpha on H2O2 production in cultured neuroblastoma cells by enhanced luminol-dependent chemiluminiscence (ECL). / Br Homeopatic J 1997, 86:67-2. CrossRef
  31. Paul D, Lipton A, Klinger I: Serum factor requirements of normal an simian virus 40-transformed 3T3 mouse fibroblasts. / Proc Nat Acad Sci USA 1971, 68:645-52. CrossRef
  32. Niebauer GW, Dorcsi M, Pfeil L: Die Wirkung von hom?opathischen Arnica D und Actihaemyl auf die Wundheilung im Tierexperiment. / Prakt Tierarzt 1980, 61:128-32.
  33. Rao SG, Udupa AL, Udupa SL, Rao PGM, Rao G, Kulkarni DR: Calendula and Hypericum: two homeopathic drugs promoting wound healing in rats. / Fitoterapia 1991, 62:508-10.
  34. Conforti A, Bellavite P, Bertani S, Chiarotti F, Menniti-Ippolito F, Raschetti R: Rat models of acute inflammation: a randomized controlled study on the effects of homeopathic remedies. / BMC Complement Altern Med 2007, 7:1. CrossRef
  35. Wagner S, Suter A, Merfort I: Skin penetration studies of Arnica preparations and of their sesquiterpene lactones. / Planta Med 2004, 70:897-03. CrossRef
  36. Tekko IA, Bonner MC, Bowen RD, Williams AC: Permeation of bioactive constituents from Arnica montana preparations through human skin in vitro. / J Pharm Pharmacol 2006, 58:1167-176. CrossRef
  37. Preethi KC, Kuttan G, Kuttan R: Antioxidant potential of Calendula officinalis flowers in vitro and in vivo. / Pharm Biol 2006, 44:691-97. CrossRef
  38. Thring TSA, Hili P, Naughton DP: Anti-collagenase, anti-elastase and anti-oxidant activities of extracts from 21 plants. / BMC Complement Altern Med 2009, 9:27. CrossRef
  39. Mathew A, Taranalli AD, Torgal SS: Evaluation of anti-inflammatory and wound healing activity of Genziana lutea rhizome extracts in animals. / Pharm Biol 2004, 42:8-2. CrossRef
  40. J?ggi R, Würgler U, Grandjean F, Weiser M: Dual inhibition of 5-lipoxygenase/cyclooxygenase by a reconstituted homeopathic remedy; possible explanation for clinical efficacy and favourable gastrointestinal tolerability. / Infamm Res 2004, 53:150-57. CrossRef
  41. Cazin JC, Cazin M, Gaborit JL, Chaoui A, Boiron J, Belon P, Cherruault Y, Papapanayotou C: A study of the effect of decimal and centesimal dilutions of arsenic on the retention and mobilization of arsenic in the rat. / Human Toxicol 1987, 6:315-20. CrossRef
  42. Belon P, Cumps J, Ennis M, Mannaioni PF, Sainte-Laudy J, Roberfroid M, Wiegant FAC: Inhibition of human basophil degranulation by successive histamine dilutions: Results of a European multi-centre trial. / Inflamm Res 1999,48(Suppl.1):S17-S18. CrossRef
  43. W?lchli C, Baumgartner S, Bastide M: Effect of low doses and high homeopathic potencies in normal and cancerous human lymphocytes: An in vitro isopathic study. / J Altern Complement Med 2006, 12:421-27. CrossRef
  44. Witt CM, Bluth M, Albrecht H, Weisshuhn TER, Baumgartner S, Willich SN: The in vitro evidence for an effect of high homeopathic potencies- A systematic review of the literature. / Complement Therapies in Med 2007, 15:128-38. CrossRef
  45. The pre-publication history for this paper can be accessed here:http://www.biomedcentral.com/1472-6882/12/100/prepub
  
• 作者单位：Katarina Hostanska (1)
  Matthias Rostock (1) (2)
  Joerg Melzer (1)
  Stephan Baumgartner (3) (4) (5)
  Reinhard Saller (1)
  
  1. Institute for Complementary Medicine, University Hospital Zurich, Raemistrasse 100, Zurich, 8091, Switzerland
  2. University Medical Center Hamburg-Eppendorf, University Cancer Center Hamburg, Hubertus Wald Tumor Center, Martinistrasse 52, Hamburg, 20246, Germany
  3. Institute of Complementary Medicine KIKOM, University of Bern, Imhoof-Pavillon, Insel-Spital, Bern, 3010, Switzerland
  4. Center for Integrative Medicine, University of Witten/Herdecke, Gerhard-Kienle-Weg 4, Herdecke, 58313, Germany
  5. Society for Cancer Research, Kirschweg 9, Arlesheim, 4144, Switzerland
  

文摘

Background Drugs of plant origin such as Arnica montana, Calendula officinalis or Hypericum perforatum have been frequently used to promote wound healing. While their effect on wound healing using preparations at pharmacological concentrations was supported by several in vitro and clinical studies, investigations of herbal homeopathic remedies on wound healing process are rare. The objective of this study was to investigate the effect of a commercial low potency homeopathic remedy Similasan? Arnica plus Spray on wound closure in a controlled, blind trial in vitro. Methods We investigated the effect of an ethanolic preparation composed of equal parts of Arnica montana 4x, Calendula officinalis 4x, Hypericum perforatum 4x and Symphytum officinale 6x (0712-), its succussed hydroalcoholic solvent (0712-) and unsuccussed solvent (0712-) on NIH 3T3 fibroblasts. Cell viability was determined by WST-1 assay, cell growth using BrdU uptake, cell migration by chemotaxis assay and wound closure by CytoSelect ?Wound Healing Assay Kit which generated a defined “wound field- All assays were performed in three independent controlled experiments. Results None of the three substances affected cell viability and none showed a stimulating effect on cell proliferation. Preparation (0712-) exerted a stimulating effect on fibroblast migration (31.9%) vs 14.7% with succussed solvent (0712-) at 1:100 dilutions (p-lt;-.001). Unsuccussed solvent (0712-) had no influence on cell migration (6.3%; p-gt;-.05). Preparation (0712-) at a dilution of 1:100 promoted in vitro wound closure by 59.5% and differed significantly (p-lt;-.001) from succussed solvent (0712-), which caused 22.1% wound closure. Conclusion Results of this study showed that the low potency homeopathic remedy (0712-) exerted in vitro wound closure potential in NIH 3T3 fibroblasts. This effect resulted from stimulation of fibroblasts motility rather than of their mitosis.