Expression of Critical Sulfur- and Iron-Oxidation Genes and the Community Dynamics During Bioleaching of Chalcopyrite Concentrate by Moderate Thermophiles

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• 作者：Dan Zhou ; Tangjian Peng ; Hongbo Zhou ; Xueduan Liu ; Guohua Gu…
• 刊名：Current Microbiology
• 出版年：2015
• 出版时间：July 2015
• 年：2015
• 卷：71
• 期：1
• 页码：62-69
• 全文大小：574 KB
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  25.Zhang R,
• 作者单位：Dan Zhou (1)
  Tangjian Peng (1)
  Hongbo Zhou (1) (2)
  Xueduan Liu (1) (2)
  Guohua Gu (1) (2)
  Miao Chen (3)
  Guanzhou Qiu (1) (2)
  Weimin Zeng (1) (2) (3)
  
  1. School of Minerals Processing and Bioengineering, Central South University, Changsha, China
  2. Key Laboratory of Biometallurgy, Ministry of Education, Changsha, 410083, Hunan, China
  3. CSIRO Process Science and Engineering, Clayton, VIC, Australia
  
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Life Sciences
  Microbiology
  Biotechnology
  
• 出版者：Springer New York
• ISSN：1432-0991

文摘

Sulfate adenylyltransferase gene and 4Fe-S ferredoxin gene are the key genes related to sulfur and iron oxidations during bioleaching system, respectively. In order to better understand the bioleaching and microorganism synergistic mechanism in chalcopyrite bioleaching by mixed culture of moderate thermophiles, expressions of the two energy metabolism genes and community dynamics of free and attached microorganisms were investigated. Specific primers were designed for real-time quantitative PCR to study the expression of these genes. Real-time PCR results showed that sulfate adenylyltransferase gene was more highly expressed in Sulfobacillus thermosulfidooxidans than that in Acidithiobacillus caldus, and expression of 4Fe-S ferredoxin gene was higher in Ferroplasma thermophilum than that in S. thermosulfidooxidans and Leptospirillum ferriphilum. The results indicated that in the bioleaching system of chalcopyrite concentrate, sulfur and iron oxidations were mainly performed by S. thermosulfidooxidans and F. thermophilum, respectively. The community dynamics results revealed that S. thermosulfidooxidans took up the largest proportion during the whole period, followed by F. thermophilum, A. caldus, and L. ferriphilum. The CCA analysis showed that 4Fe-S ferredoxin gene expression was mainly affected (positively correlated) by high pH and elevated concentration of ferrous ion, while no factor was observed to prominently influence the expression of sulfate adenylyltransferase gene.