Expression of HIV-encoded microRNA-TAR and its inhibitory effect on viral replication in human primary macrophages

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• 作者：Li Li ; Haimin Feng ; Qin Da ; Honglin Jiang ; Lang Chen ; Linlin Xie…
• 刊名：Archives of Virology
• 出版年：2016
• 出版时间：May 2016
• 年：2016
• 卷：161
• 期：5
• 页码：1115-1123
• 全文大小：2,048 KB
• 刊物类别：Biomedical and Life Sciences
• 刊物主题：Biomedicine
  Virology
  Medical Microbiology
  Infectious Diseases
  
• 出版者：Springer Wien
• ISSN：1432-8798
• 卷排序：161

文摘

A number of virus-encoded microRNAs have been shown to play important roles in virus replication and virus-host interactions, although the expression and function of miR-TAR-3p derived from the human immunodeficiency virus type 1 (HIV-1) TAR element remain controversial. In this study, miR-TAR-3p was detected in human peripheral blood monocyte-derived macrophages (MDMs) infected by HIV-1. Overexpression of miR-TAR-3p impaired viral replication, while inhibition of miR-TAR-3p enhanced it. Additionally, miR-TAR-3p repressed viral transcription and replication by targeting the TAR element in the HIV-1 5’-LTR in a sequence-specific manner. These results confirm the presence of miR-TAR-3p in HIV-1-infected MDMs and suggest that its function might be used as a mechanism to modulate HIV-1 replication through the expression of a negative regulatory factor.